تأثير قطر البويضة في إنتاج الأجنة خارج الرحم داخل الفصل التناسلي و خارجه في المعز السوري

الملخص EN

This research was conducted at the laboratories of National Commission for Biotechnology in Damascus during 2013 to study the effect of oocytes diameter in and outside of reproductive season in in vitro embryo production of Syrian goats and determine the rate of survivability of post vitrified-thawed embryos.

Ovaries of slaughtered goats collected and placed immediately into PBS at 37 ̊C were transported to the laboratory within 2 hours.

Each ovary was sliced with a sharp tool and washed with warm medium of PBS to rinse and collect oocytes.

Collected oocytes were classified according to the diameter into four groups (<100, 100-110, >110-120, > 120 µm).

Morula embryos were vitrified with DMSO 15% + EG 15% and evaluated to determine post thawed survivability.

Data were layouted according to GLM using SPSS 17, and F-Test was used to compare among means.

Results indicated that slicing technique allowed to collect 40oocyte/ female with overall rates of oocyte maturation, fertilization, cleavage and survivability of post vitrified- thawed embryos were 42.54%, 29.35%, 28.8% 47.17%, respectively.

Analysis of variance showed significant effects (P<0.01) for oocyte diameter on studied traits.

Matured oocytes collected with diameter less than 110 µm in and outside of reproduction season did not have the ability to divide and develop to give embryos.

It was concluded that slaughtered animals house can be a good source of low-cost for the production of goats' embryos in vitro by oocytes with diameter >110 µm regardless of reproduction season and it is recommended that more investigations are needed to develop the competence of oocytes with < 110 µm to enable embryo production ex vivo.