التقدير الطيفي للميزالازين بالأقتران مع 4,2- ثنائي نيتروأنيلين المؤزوت

الملخص EN

A quick ,convenient and sensitive method has been developed for the determination of microgram amounts of mesalazine in its pure form and pharmaceutical preparations .

The method is based on the coupling reaction of the drug with diazotized 2,4 - dinitroaniline reagent in an alkaline medium to produce an intense orange coloured water soluble and stable azo dye which exhibits a maximum absorption at 500 nm.

Beer's law was obeyed over the concentration range 1 - 8 μg/ml with molar absorptivity of 1.199 ×104 L.mol-1.cm-1 and Sandell's sensitivity index of 0.0119 g/cm2.

The limit of detection is 0.1011 μg/ml while the limit of quantitation is 0.3371μg/ml .

The method shows high accuracy (average recovery 99.6 %) and precision(relative standard division(RSD) is less than 2%.The suggested procedure was applied for determination of mesalazine without any interference from common pharmaceutical excipients .The proposed method is successfully compared with the official method