الكشف عن ظاهرة استشعار النصاب Quorum sensing في جرثومة Pseudomonas aeruginosa باستخدام بعض السلالات الكاشفة القياسية

الملخص EN

This research aimed to study Quorum Sensing (QS) in Pseudomonas aeruginosa isolated from burn wound infections and hospital environment in comparison with environmental isolates, using the indicator strains (Biosensors) that detect the autoinducer signals (N-acyl homoserine lactones AHLs).

The Biosensors used in this study were: Agrobacterium tumefaciens KYC55 which produce -galactosidase that trun X-Gal to blue color in the presence of AHLs and Chromobacterium violaceum CV026 which produce violacein pigment in the presence of AHLs.

Detection of AHLs in nineteen P.

aeruginosa isolates of different sources (patients, ward, water & soil) revealed that all of them were AHLs producers using KYC55 while CV026 detect AHLs in 26.3% of isolates.