Purification and characterization of lipase produced by Pseudomonas aeruginosa isolated from some soils of Basrah Governorate

المؤلفون المشاركون

al-Kabi, Hana Jafar Jabbar
al-Hamdani, Maytham Ayyub

المصدر

مجلة جامعة الكوفة لعلوم الحياة : مجلة علمية محكمة

الناشر

جامعة الكوفة كلية العلوم قسم علوم الحياة

تاريخ النشر

2018-12-31

دولة النشر

العراق

عدد الصفحات

8

التخصصات الرئيسية

الأحياء

الموضوعات

الملخص الإنجليزي

The present study aimed to Purification and characterization of lipase produced by the isolate Pseudomonas aeruginosa.

The results showed that crude lipase was purified by using the precipitation step of several concentrations ratios of cold acetone.

The concentration ratio 4:1 gave the best specific activity 6 U/mg of protein.

The subsequent purification step using gel filtration was showed that specific activity reached to 40.5U/mg of protein.

The gel electrophoresis (SDS-PAGE) of lipase purity were determined.

the result showed there is an appearance of a single protein band predominant in all purification steps, While The molecular weight of purified lipase was 68KDa

نوع البيانات

أوراق مؤتمرات

رقم السجل

BIM-910920

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

al-Kabi, Hana Jafar Jabbar& al-Hamdani, Maytham Ayyub. 2018-12-31. Purification and characterization of lipase produced by Pseudomonas aeruginosa isolated from some soils of Basrah Governorate. International Scientific Conference (3rd : 2018 : Najaf, Iraq). . Special issue (2018), pp.138-145.Najaf Iraq : University of Kufa Faculty of Science Department of live Sciences.
https://search.emarefa.net/detail/BIM-910920

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

al-Kabi, Hana Jafar Jabbar& al-Hamdani, Maytham Ayyub. Purification and characterization of lipase produced by Pseudomonas aeruginosa isolated from some soils of Basrah Governorate. . Najaf Iraq : University of Kufa Faculty of Science Department of live Sciences. 2018-12-31.
https://search.emarefa.net/detail/BIM-910920

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

al-Kabi, Hana Jafar Jabbar& al-Hamdani, Maytham Ayyub. Purification and characterization of lipase produced by Pseudomonas aeruginosa isolated from some soils of Basrah Governorate. . International Scientific Conference (3rd : 2018 : Najaf, Iraq).
https://search.emarefa.net/detail/BIM-910920