Antioxidant role of Lactobacillus sp isolated from honey bee against histological effects of Ochratoxin in vivo

الملخص EN

In this study One hundred honey bee worker were collected from two sites in ALDiwanyia Governorate include Eastern Hamza and AL-Daghara city during April 2017.

to detect antioxidant role of some bacteria isolated from honey bee gut workers against Ochratoxin.

Based on biochemical tests, the results show that out of one hundred samples of honey bee workers were screened, only Seventy isolates were isolated ,and the genera lactic acid bacteria ( Lactobacillus kunkeei ,lactobacillus plantrum and lactococus lactis) were highly abundant in honey bee gut .

we examined protective role of Lactobacillus kunkeei in reduce histological effects of Ochratoxin A, the results showed treated animals with L.

kunkeei.

mixed with OTA showing not found any histopathological changes in liver and kidney.while treated animals with OTA only appear several pathological changes ,in kidney tissue this changes include :vascular congestion , dystrophy and the disappearance of the glomerulus in addition to cell necrosis of glomerulus.and cell necrosis, vascular congestion and loss of normal structure of liver.

Lactobacillus kunkeei isolated from honey bee appear good protective role against histological effect of OTA and may safely improve immune responsiveness.

This is the first report for detection of protective role of honey bee bacteria against OTA .and this bacteria appear good role in degradation of mycotoxin.therfore we recommended for detection of LD50 and LD90 for this bacteria and use as supplement drugs for mycotoxicosis