تطهير خميرة Candida Candida باستخدام بلازما اللاركون غير الحرارية المنتجة عند الضغط الجوي

الملخص EN

The sterilization of candida albicans yeast was done by using atmospheric pressure non thermal argon plasma, the plasma jet system was used for this purpose with following operations conditions: the discharge voltage was (8) kV, with frequency (22) kHz and argon gas flow (3) l/min.

This study included obtaining clinical isolate of C.albicans yeast which diagnosed initially according to its colonies appearance on SDA as a a smooth, creamy and mucosa colonies , while it characterized by a production of Pseudohyphae and the presence of chlamidospores on CMA, also it has the ability to grow at 45 C , positive to germ tube production , Gram positive oval shape cells .

This study also included investigation the inhibitory effect of cold plasma at different periods of time on the yeast isolate growth, where the results obtained did not show any effect of cold plasma on the growth of the yeast during the first stage of exposure in seconds comparing to control while it was noted a decrease in growth when increasing the exposure time to minute during the second phase of treatment till yeast growth entirely down to zero when the exposure period reached 10 minutes, also This study was Investigated the inhibiting efficiency of cold plasma exposure on the ability of C.albicans yeast isolate to produced germ tube before and after exposure to a nonthermal plasma where the results showed increased in the inhibition percentage of yeast germination along with increased time of exposure from 59 % at exposure time 6 min down to 96% when the exposure time 9 min compared with the control,but the exposure to a non-thermal plasma did not demonstrated demonstrated any morphological changes in yeast cells and their acceptance of staining compared to the control not treated with cold plasma