التحري عن بعض المركبات الفينولية في عسل النحل بتقنية كروموتوغرافيا السائل عالي الإداء و تأثيره التثبيطي في مسبب مرض تقرح ساق الروبينيا

الملخص EN

The study involved identification of some phenolic compounds for two kinds of honey bees in two positions by high performance liquid chromotography technique (HPLC).

The results showed identical the retention time values of identificated compounds with the standard retention time values of the same compounds at the two kind of bees honey for two positions and the retention time values were for gallic acid, hydroxy cinnamic acid, Resorcinol ( 1.02 , 1.46 , 1.18 ) minutes respectively, it was Identical with standard retention time values of the same compounds in the sample of non-adult bees honey / Danadan position, while repeated identification of the compounds hydroxy cinnamic acid , Resorcinol as well as Coumarin and Salsalic acid in the sample of adult bees honey and for the same position , the values of retention time for Coumarin and Salsalic acid ( 1.67 , 1.38 ) minutes respectively.

Then the same phenolic compounds whom identifacated previously in adult honey sample / Danadan position were repeated appear in the sample of non-adult honey / Agri & forestry college position additional Quercetin which its retention time value was ( 1.33 ) minutes and which identical with standard retention time value of the same compound The results of identification adult honey sample / Agri & forestry college position showed repeating appear of the compounds hydroxy cinnamic acid , Resorcinol , Salsalic acid and Quercetin and whom its retention time values identical with standard retention time values of the same compound.

Qualitative identification for the phenolic compounds showed differ with ratios as difference of positions and kinds, Resorcinol showed maximum ratio ( 47.23%) in non-adult honey / Danadan position sample while Coumarin showed a minimum ratio ( 2.23%) in the same kind of honey an the same position .Gallic acid only identificated in nonadult honey / Danadan position sample and and its rasio was ( 6.41% ).

Bioassay results of honey samples showed inhibition the growth of Nattrassia mangifera which was the causal agent of Robinia pseudoacacia stem canker, Inhibition ratio was 100% at the concentration 100% of adult bees honey.