Detection of the level fetal bovine serum for growth and vitality of fibroblasts cells of frozen chicken embryos

العناوين الأخرى

الكشف عن مستوى مصل الدم المناسب لنمو و حيوية الخلايا الليفية لأجنة الدجاج المجمدة

المؤلفون المشاركون

Guixue, Zhang
Khalid, Ahmad
Khudayr, Najm
Abd al-Aziz, Amr
Abd al-Mutaal, Hibah
Fawzi, Hanan
Hatim, Nuha
Muhammad, Ibtahal

المصدر

مجلة تكريت للعلوم الزراعية

الناشر

جامعة تكريت كلية الزراعة

تاريخ النشر

2018-12-31

دولة النشر

العراق

عدد الصفحات

9

التخصصات الرئيسية

علم الحيوان

الموضوعات

• التشريح
• الأجنحة
• الطب البيطري
• الدجاج المجمد
• مصل الدم
• الأرومات الليفية

الملخص الإنجليزي

The culture technology has prolificated effectively within a century, a variety of culture media has been designed.

The chicken embryo is an excellent model for studying embryology and the production of pharmaceutical proteins in transgenic chickens.

In addition, chicken stem cells have the potential for differentiation and proliferation and emerged as an attractive tool for various cell-based technologies.

The bank had stocks of 90 cryogenically-preserved vials each containing 4~5×106 cells (Frozen tissue embryonic).

The cells were cultured individually in three concentrations of FBS like 3.5%, 7%, and 10%.

The cells were morphologically consistent with the fibroblasts and the growth curve was sigmoidal.

And the result shows that 10% FBS concentration was best for fibroblast culture in vitro.

In this study the fibroblasts have been established successfully from frozen chicken embryos by the method of enzymatic digestion.

نوع البيانات

أوراق مؤتمرات

رقم السجل

BIM-922181

• APA
• MLA
• AMA