Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques

العناوين الأخرى

تحديد المكورات العنقودية الذهبية المقاومة للمثيسيلين في الدواجن و عمال حقول الدواجن في دهوك-العراق باستخدام الطرق التقليدية و تفاعل البلمرة المتسلسل

المؤلفون المشاركون

al-Assafi, Mahdi Salih
Haddu, Hushyar Abd al-Jabbar
Abd al-Rahman, Ibtisam Salih

المصدر

Iraqi Journal of Veterinary Sciences

العدد

المجلد 34، العدد 1 (30 يونيو/حزيران 2020)، ص ص. 15-22، 8ص.

الناشر

جامعة الموصل كلية الطب البيطري

تاريخ النشر

2020-06-30

دولة النشر

العراق

عدد الصفحات

8

الموضوع الرئيسي

الطب البيطري

الملخص EN

Livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) has become a global public health concern. The purpose of this study was to investigate the prevalence rates of MRSA infection amongst broiler chickens and broilers farm workers. The total samples used in this study were 306. Cloacal swab samples from 231 broilers and nasal swab samples from 75 broilers farm workers were collected from five farms in Duhok city, Iraq. Isolation and identification of MRSA isolates were carried out and the antibiotic susceptibility were screened. Molecular characterization of all isolates was performed by using polymerase chain reaction (PCR) technique to detect the mecA gene. S. aureus was detected among 84% (63/75) of the farms workers samples and among 84.8% (196/231) of the broiler's samples. The S. aureus isolated from farm workers and broilers appeared resistant to oxacillin 28.6% (18/63), and 32.1% (63/196), respectively. MRSA colonization in farm workers and broilers was 24% (18/75) and 27.3% (63/231) respectively. The S. aureus isolates showed the most resistant to chloramphenicol and the least resistant to vancomycin. The results of the PCR assays revealed that 85.7% (12/14) of S. aureus isolates from farm workers and 44.4% (16/36) of S. aureus isolates from broilers were positive for the mecA gene. The direct handling of broilers by farm workers plays the important role for transport the MRSA isolates from broilers to broilers farm workers.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

al-Assafi, Mahdi Salih& Haddu, Hushyar Abd al-Jabbar& Abd al-Rahman, Ibtisam Salih. 2020. Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques. Iraqi Journal of Veterinary Sciences،Vol. 34, no. 1, pp.15-22.
https://search.emarefa.net/detail/BIM-948781

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

al-Assafi, Mahdi Salih…[et al.]. Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques. Iraqi Journal of Veterinary Sciences Vol. 34, no. 1 (2020), pp.15-22.
https://search.emarefa.net/detail/BIM-948781

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

al-Assafi, Mahdi Salih& Haddu, Hushyar Abd al-Jabbar& Abd al-Rahman, Ibtisam Salih. Detection of methicillin-resistant staphylococcus aureus in broiler and broilers farm workers in Duhok, Iraq by using conventional and PCR techniques. Iraqi Journal of Veterinary Sciences. 2020. Vol. 34, no. 1, pp.15-22.
https://search.emarefa.net/detail/BIM-948781

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references : p. 20-22

رقم السجل

BIM-948781