Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province

العناوين الأخرى

تشخيص الإصابة بفيروس الريو في أمهات فروج اللحم باستخدام تقنية تفاعل البلمرة المتسلسل في محافظة أربيل

المؤلف

Ishaq, Fanar Ablahad

المصدر

Iraqi Journal of Veterinary Sciences

العدد

المجلد 34، العدد 1 (30 يونيو/حزيران 2020)، ص ص. 77-81، 5ص.

الناشر

جامعة الموصل كلية الطب البيطري

تاريخ النشر

2020-06-30

دولة النشر

العراق

عدد الصفحات

5

الموضوع الرئيسي

الطب البيطري

الملخص EN

Avian reoviruses can infect birds without any clinical signs of infection, the infection may associate with different manifestations including viral arthritis/tenosynovitis and malabsorption syndrome. The objective of this study was to use advance methods representing by molecular methods (RT-PCR, RT-qPCR) in the diagnosis of ARV infection in broiler breeders' flocks. A 4 flocks of broiler breeders (ROSS breed) 39 weeks age with approximately 10% morbidity rate due to Avian Reovirus (ARV). The clinical examination of 16 infected birds revealed unilateral lameness and swelling of hock joint. Blood samples were collected from wing vein of infected birds. Sera were tested for antibodies titer against ARV and Mycoplasma synoviae (MS). 5 of 16 positive samples were selected randomly for amplification by RT-PCR and RT-qPCR. The results showed in postmortem examination of infected birds, unilateral arthritis with visible joint lesions. Antibodies titer measured by ELISA in the sera of birds after 4 and 20 weeks of infection with ARV was positive and high. In RT- PCR 1 of 5 samples gave positive reaction for amplification while in RT-qPCR all five samples gave positive results for amplification in comparison with +ve and -ve control.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Ishaq, Fanar Ablahad. 2020. Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province. Iraqi Journal of Veterinary Sciences،Vol. 34, no. 1, pp.77-81.
https://search.emarefa.net/detail/BIM-948790

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Ishaq, Fanar Ablahad. Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province. Iraqi Journal of Veterinary Sciences Vol. 34, no. 1 (2020), pp.77-81.
https://search.emarefa.net/detail/BIM-948790

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Ishaq, Fanar Ablahad. Diagnosis of reovirus infection in broiler breeders flocks by using PCR technique in Erbil province. Iraqi Journal of Veterinary Sciences. 2020. Vol. 34, no. 1, pp.77-81.
https://search.emarefa.net/detail/BIM-948790

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references : p. 81

رقم السجل

BIM-948790