تأثير بعض الأحماض الأمينية و الفيتامينات في إكثار نباتات الهيل Elettaria cardamomum Maton. خارج الجسم الحي In vitro

الملخص EN

Two experiments were carried out to propagate cardamom plants Elettaria cardamomum Maton.

In vitro, The first involved the effect of using amino acids (Glycine, Methionine, Glutamine, Aspargine and Gasaine) at concentrations (100, 200, 300, 400, 500)mg/L, in addition the control treatment (2mg/L Glysin according to MS 1962 formula).

The second experiment studied the effect of adding some vitamins group B (Thiamin, Nicotinic acid and Pyridoxine) at concentrations (10, 20, 30, 40) mg/L, as well as the control treatments (0.1, 0.5, 0.5) mg/L respectively each of them (according to MS 1962 formula).

The results, after 8 weeks of culturing, indicated that adding Glycine 100 mg/L, Methionine 100mg/L, Gasain 100mg/L, Aspargine 400 mg/L to the media had a significant increase in number of shoots (9.44, 11.20, 12.33, 11.6 shoot/explant) respectively in comparison with control (2mg/L Glycine) giving 6.73 shoot/explant.

Whereas, adding of Aspargine in studied concentration caused a significant increase in shoot length, number of leaves, number of roots and root length as compared with control treatment.

As for the effect of Vitamins, the results showed that increasing of Thiamin concentration up to 10 mg/L caused a significant increase in number of shoots (8.14 shoot/explant) and up to 20 mg/L a significant increase in number of leaves (3.54 leaf/explant) in comparison with 4.5 shoot/explant and 2.74 leaf/explant in control treatment respectively.

While adding 30 mg/L of Pyridoxine caused a significant increase in number of shoots (9.60 shoot/explant) and number of leaves (3.33 leaf/explant) in comparison with control treatment (8.67 , 2.90) respectively.