التشخيص الجزيئي لفايروس الورقة المروحية للعنب بطريقة النسخ العكسي لتفاعل البلمرة المتسلسل (RT-PCR)‎

الملخص EN

This study was conducted to molecular diagnostics of Grapevine fanleaf virus (GFLV) in leaf samples of grapevine were collected from the grapevine yards in Salahuddin governorate, where grapevines are commonly grown in Iraq.

The virus was detected in the samples by using SuperScriptTM III RT kit and pair of specific primers GFLV2231F and GFLV2533R was used to amplify fragments of the coat protein gene (CP) in rapid direct one tube RT-PCR.