دراسة بعض الظروف المثلى للفعالية البيولوجية لبكتريا Bacillus subtilis في تحلل السليلوز

الملخص EN

This study aimed to use biological treatment of cellulosic wastes, that cause environmental pollution, by using local bacterial isolate of Bacillus subtilis which is efficient for cellulolytic activity.

Optimum conditions for detection of bacterial isolate activity, which represented by pH, cellulose concentration and temperature to show the effect of condtions on cellulolytic efficiency.

The cellulolytic activity of bacterial isolate was tested by growing the bacterial isolate in a range of pH values 6.

0, 6.

5, 7.

0, 7.

5 and 8.

0, a range of cellulose concentrations 0.

3, 0.

6, 1.

0, 1.

5 and 2.

0% and under effect of different temperatures 37, 45, 55 and 65 C°.

The optimum conditions were determined by measuring the bacterial growth by using optical density (orbance) at wave length 600 nm, to know the bacterial growth, glucose concentration was estimated and the ratio of cellulose consumption in all samples was calcutaed.

The optimum conditions were founded at pH 7.

0, cellulose concentration 1.

5 %, and at 37 C°.