تشخيص عزلات محلية من بكتيريا Lactobacillus plantarum و تقويم كفاءتها في مكافحة مرض ذبول فيوزاريوم على البندورة-الطماطم

الملخص EN

Three isolates of lactic acid bacteria, Lactobacillus plantarum were isolated from tomato rhizosphere of the Plant Protection Department fields (Tikrit University), Al-alam and Al-dejial regions, Salah Aldin Governorate, Iraq.

The isolates were identified morphologically and biochemically and were given the code numbers 1050, 1051 and 1052.

This identification was confirmed by molecular tests based on 16S rRNA sequencing, and were registered in NCBI as MN880156.1, MN880158.1 and MN880160.1, respectively.

The results showed that L.

plantarum 1052 and L.

plantarum 1051 in presence of the pathogenic fungus F.

oxysporum f.

sp.

lycopersici gave maximum shoot and root dry weight, chlorophyll content and plant height, and were significantly superior to other treatments and to the pathogenic fungus treatment alone.

The highest resistance induction indicators, including glucanase, chitinase and peroxidase produced by L.

plantarum 1052, and yielded 2.87, 4.13 and 5.84 units/mg protein, whereas the lowest values were 0.71, 1.33 and 1.02 unit/mg protein in uninfected plants, respectively.

L.

plantarum 1052 showed minimum infection rate and severity of 18.62 and 15.44%, respectively, and plant productivity reached 4981.26 g/plant, as compared to the lowest productivity of 716.56 g/plant in the presence of the pathogenic fungus alone.