Fungal transformational studies on β-Caryophyllene and biological evaluation of the resulting metabolites

العناوين الأخرى

الدراسات التحويلية الفطرية على β-Caryophyllene و التقييم البيولوجي للأيضات الناتجة

مقدم أطروحة جامعية

al-Dulaymi, Qutaybah Ghazi Ibrahim

مشرف أطروحة جامعية

al-Najdawi, Manal
Muhammad, Yasin Muhammad

الجامعة

جامعة الإسراء

الكلية

كلية الصيدلة

دولة الجامعة

الأردن

الدرجة العلمية

ماجستير

تاريخ الدرجة العلمية

2020

الملخص الإنجليزي

Microbial transformations are organic reactions, catalyzed by microorganisms such as fungi.

They are often the key steps in the synthesis of many drug molecules, and natural products and their derivatives.

This technique has also been widely employed for the synthesis of new analogues of terpenoids.

During this work, a comparative microbial transformational study of selected terpenoid (β-caryophyllene) was carried out by various fungi to produce a new metabolite with interesting biological activities.

The transformed product was purified by using chromatographic procedures, and its structure was elucidated with the help of the spectroscopic techniques.

The transformed product was also evaluated for their biological activities, such as antioxidant and β-carotene bleaching assays.

β-Caryophyllene ((1R,4E,9S)-4,11,11-trimethyl-8-methylidenebicyclo[7.2.0]undec- 4ene, C15H24) (1), a bicyclicsesquiterpeneisolatedfromtheessentialoilsofthestems and the flowers of Syzgium aromaticum (cloves), and the essential oils of Cannabis sativa and Salvia rosmarinus (rosemary), has been shown to have anti-cancer, antioxidant, anti-inflammatoryand anti-bacterial activities.

Microbial transformation of β-caryophyllene (44), by suspended cell-cultures of the plant pathogenic fungus Aspergillus niger, resulted in the production of one metabolite.

The metabolite was identified as (3a,11a,E)-9-methyl-4- methylene3a,4,7,10,11,11a-hexahydro-2H-furo[3,2-c]oxecine2,6(3H)-dione (45), by differentspectroscopicmethods.Compound2wasfoundtobenew.

The antioxidant activity of β-caryophyllene (44), metabolite 45 and the standard was also studied on the basis of the radical scavenging effect of the stable DPPH free radical.

Compound 45 showed appreciable antioxidant activity.

التخصصات الرئيسية

علم الصيدلة

الموضوعات

• التقييم البيولوجي
• المستقلبات الفطرية
• الكاريوفيلين

عدد الصفحات

65

قائمة المحتويات

• APA
• MLA
• AMA

لغة النص

الإنجليزية

نوع البيانات

رسائل جامعية

رقم السجل

BIM-985113