Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing
المؤلفون المشاركون
Sung, Jung-Suk
Cho, Sungbo
Koh, Hyun Sook
Kim, Donghee
Park, Hyo Eun
Kim, Jae Young
المصدر
العدد
المجلد 2011، العدد 2011 (31 ديسمبر/كانون الأول 2011)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2011-05-17
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Stem cells are useful for cell replacement therapy.
Stem cell differentiation must be monitored thoroughly and precisely prior to transplantation.
In this study we evaluated the usefulness of electric cell-substrate impedance sensing (ECIS) for in vitro real-time monitoring of neural differentiation of human mesenchymal stem cells (hMSCs).
We cultured hMSCs in neural differentiation media (NDM) for 6 days and examined the time-course of impedance changes with an ECIS array.
We also monitored the expression of markers for neural differentiation, total cell count, and cell cycle profiles.
Cellular expression of neuron and oligodendrocyte markers increased.
The resistance value of cells cultured in NDM was automatically measured in real-time and found to increase much more slowly over time compared to cells cultured in non-differentiation media.
The relatively slow resistance changes observed in differentiating MSCs were determined to be due to their lower growth capacity achieved by induction of cell cycle arrest in G0/G1.
Overall results suggest that the relatively slow change in resistance values measured by ECIS method can be used as a parameter for slowly growing neural-differentiating cells.
However, to enhance the competence of ECIS for in vitro real-time monitoring of neural differentiation of MSCs, more elaborate studies are needed.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Park, Hyo Eun& Kim, Donghee& Koh, Hyun Sook& Cho, Sungbo& Sung, Jung-Suk& Kim, Jae Young. 2011. Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing. BioMed Research International،Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990142
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Park, Hyo Eun…[et al.]. Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing. BioMed Research International No. 2011 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-990142
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Park, Hyo Eun& Kim, Donghee& Koh, Hyun Sook& Cho, Sungbo& Sung, Jung-Suk& Kim, Jae Young. Real-Time Monitoring of Neural Differentiation of Human Mesenchymal Stem Cells by Electric Cell-Substrate Impedance Sensing. BioMed Research International. 2011. Vol. 2011, no. 2011, pp.1-8.
https://search.emarefa.net/detail/BIM-990142
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-990142
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر