Inhibition of Activity of GABA Transporter GAT1 by δ-Opioid Receptor
المؤلفون المشاركون
Schwarz, Wolfgang
Pu, Lu
Xu, Nanjie
Xia, Peng
Gu, Quanbao
Ren, Shuanglai
Fucke, Thomas
Pei, Gang
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-12، 12ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-12-25
دولة النشر
مصر
عدد الصفحات
12
التخصصات الرئيسية
الملخص EN
Analgesia is a well-documented effect of acupuncture.
A critical role in pain sensation plays the nervous system, including the GABAergic system and opioid receptor (OR) activation.
Here we investigated regulation of GABA transporter GAT1 by δOR in rats and in Xenopus oocytes.
Synaptosomes of brain from rats chronically exposed to opiates exhibited reduced GABA uptake, indicating that GABA transport might be regulated by opioid receptors.
For further investigation we have expressed GAT1 of mouse brain together with mouse δOR and μOR in Xenopus oocytes.
The function of GAT1 was analyzed in terms of Na+-dependent [3H]GABA uptake as well as GAT1-mediated currents.
Coexpression of δOR led to reduced number of fully functional GAT1 transporters, reduced substrate translocation, and GAT1-mediated current.
Activation of δOR further reduced the rate of GABA uptake as well as GAT1-mediated current.
Coexpression of μOR, as well as μOR activation, affected neither the number of transporters, nor rate of GABA uptake, nor GAT1-mediated current.
Inhibition of GAT1-mediated current by activation of δOR was confirmed in whole-cell patch-clamp experiments on rat brain slices of periaqueductal gray.
We conclude that inhibition of GAT1 function will strengthen the inhibitory action of the GABAergic system and hence may contribute to acupuncture-induced analgesia.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Pu, Lu& Xu, Nanjie& Xia, Peng& Gu, Quanbao& Ren, Shuanglai& Fucke, Thomas…[et al.]. 2012. Inhibition of Activity of GABA Transporter GAT1 by δ-Opioid Receptor. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-992675
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Pu, Lu…[et al.]. Inhibition of Activity of GABA Transporter GAT1 by δ-Opioid Receptor. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-12.
https://search.emarefa.net/detail/BIM-992675
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Pu, Lu& Xu, Nanjie& Xia, Peng& Gu, Quanbao& Ren, Shuanglai& Fucke, Thomas…[et al.]. Inhibition of Activity of GABA Transporter GAT1 by δ-Opioid Receptor. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-12.
https://search.emarefa.net/detail/BIM-992675
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-992675
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر