Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo
المؤلفون المشاركون
Shi, Junjun
Zhang, Xin
Pi, Yanbin
Zhu, Jingxian
Zhou, Chunyan
Ao, Yingfang
المصدر
العدد
المجلد 2012، العدد 2012 (31 ديسمبر/كانون الأول 2012)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2012-04-11
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
The clinical application of viral vectors for gene therapy is limited for biosafety consideration.
In this study, to promote articular cartilage repair, poly (lactic-co glycolic acid) (PLGA) nanopolymers were used as non-viral vectors to transfect rabbit mesenchymal stem cells (MSCs) with the pDC316-BMP4-EGFP plasmid.
The cytotoxicity and transfection efficiency in vitro were acceptable measuring by CCK-8 and flow cytometry.
After transfection, Chondrogenic markers (mRNA of Col2a1, Sox9, Bmp4, and Agg) of experimental cells (MSCs being transfected with BMP-4 plasmid by PLGA nanopolymers) were increased more than those of control cells (MSCs being transfected with naked BMP-4 plasmid alone).
In vivo study, twelve rabbits (24 knees) with large full thickness articular cartilage defects were randomly divided into the experimental group (MSCs being transfected with BMP-4 plasmid by PLGA nanopolymers) and the control group (MSCs being transfected with naked BMP-4 plasmid).
The experimental group showed better regeneration than the control group 6 and 12 weeks postoperatively.
Hyaline-like cartilage formed at week 12 in the experimental group, indicating the local delivery of BMP-4 plasmid to MSCs by PLGA nanopolymers improved articular cartilage repair significantly.
PLGA nanopolymers could be a promising and effective non-viral vector for gene therapy in cartilage repair.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shi, Junjun& Zhang, Xin& Pi, Yanbin& Zhu, Jingxian& Zhou, Chunyan& Ao, Yingfang. 2012. Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-997469
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shi, Junjun…[et al.]. Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo. Journal of Nanomaterials No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-997469
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shi, Junjun& Zhang, Xin& Pi, Yanbin& Zhu, Jingxian& Zhou, Chunyan& Ao, Yingfang. Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-997469
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-997469
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر