Micropropagation of zaghlol and barhy date palm cultivars using immature female inflorescence explants : effect of growth regulators balance
Other Title(s)
الإكثار الدقيق لأصناف البلح (الزغلول و البارحي) باستخدام النورات المؤنثة غير الناضجة : تأثير توازن منظمات النمو
Joint Authors
Sulayman, Said Sad Ahmad
Haykal, Raniya Muhammad Yahya
Abd al-Aziz, Asma Mahir
Hasanin, Abd Allah Abd al-Azim
Ahmad, Talat A.
Source
Zagazig Journal of Agricultural Research
Issue
Vol. 46, Issue 6 (31 Dec. 2019)13 p.
Publisher
Zagazig University Faculty of Agriculture
Publication Date
2019-12-31
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Topics
Abstract EN
The present study aimed to micropropagate two date palm cultivars (Zaghlol and Barhy) using immature female inflorescence and various growth regulators combinations.
This experiment was done in Biotechnology Laboratory, Department of Genetics, Faculty of Agriculture Zagazig University, during three consecutive seasons 2017, 2018, and 2019.
Three types of culture media were used for initiation stage, results showed that initiation medium1 (1.0 mg /l 1-Naphthaleneacetic acid (NAA) + 0.1 mg Indole-3-acetic acid (IAA) + 1.0 mg /l 2, 4-Dichlorophenoxyacetic acid (2, 4-D)) was the best medium that recorded the highest callus formation rate in both Zaghlol and Barhy cultivars.
But the Barhy cultivar showed higher response for callus formation than Zaghlol cultivar, especially for number of days to initiate callus (average mean 125.58 days compare to 279 days for Barhy and Zaghlol, respectively.
For plant regeneration stage, it was used three plant regeneration media.
Results showed that plant regeneration medium 1 (NAA 0.5 mg/l +benzyl adenine (BA) 2.0 mg/l) showed the best value for each of number of leaves (19.35), leaf length (39.75 cm) and number of days to initiate regeneration (19).
Two types of rooting media were used for root formation, it was found that rooting medium 1 (Indole-3-butyric acid (IBA) 2.0 mg/l + BA 2.0 mg/l) gave higher response than rooting medium 2 (NAA 2.0 mg/l +BA 2.0 mg/l) for almost measured criteria except root length.
Also, using rooting medium 1 decreased the number of days for root formation to 40 days compared to 110.25 days obtained from rooting medium 2.
American Psychological Association (APA)
Sulayman, Said Sad Ahmad& Abd al-Aziz, Asma Mahir& Ahmad, Talat A.& Haykal, Raniya Muhammad Yahya& Hasanin, Abd Allah Abd al-Azim. 2019. Micropropagation of zaghlol and barhy date palm cultivars using immature female inflorescence explants : effect of growth regulators balance. Zagazig Journal of Agricultural Research،Vol. 46, no. 6.
https://search.emarefa.net/detail/BIM-1021782
Modern Language Association (MLA)
Ahmad, Talat A.…[et al.]. Micropropagation of zaghlol and barhy date palm cultivars using immature female inflorescence explants : effect of growth regulators balance. Zagazig Journal of Agricultural Research Vol. 46, no. 6 (2019).
https://search.emarefa.net/detail/BIM-1021782
American Medical Association (AMA)
Sulayman, Said Sad Ahmad& Abd al-Aziz, Asma Mahir& Ahmad, Talat A.& Haykal, Raniya Muhammad Yahya& Hasanin, Abd Allah Abd al-Azim. Micropropagation of zaghlol and barhy date palm cultivars using immature female inflorescence explants : effect of growth regulators balance. Zagazig Journal of Agricultural Research. 2019. Vol. 46, no. 6.
https://search.emarefa.net/detail/BIM-1021782
Data Type
Journal Articles
Language
English
Notes
-
Record ID
BIM-1021782