Study and determination of Aryl hydrocarbon receptor with some trace elements in fuel filling station workers

Abstract EN

PollutiOn is the intrOduction Of contaminantsʹ intO the natural envirOnment that causeadverseʹ change.

Gasoline is a toxic and highly flammable liquid consists of various types ofaliphatic hydrocarbons, olefins, benzenes and aromatic hydrocarbons including toluene, xyleneand a large number of volatile compounds in addition to tetraethyl lead.

Gasoline consists ofdifferent types of aliphatic hydrocarbons, aryl compounds and some trace elements.

Traceelements are several important roles in human bodies, some are essential for enzymes reactionswhere they attract and facilitate conversion of substrate molecules to specific end products.

Aryl hydrocarbon receptor (AHR) is a receptor involved in the regulation of biologicalresponses to planar aromatic hydrocarbons.

The aim of the present study is to compare the serum AHR level in the fuel station workers(FSW) with the non-workers as a control group.

The other aim is to find out a possiblecorrelation between AHR with trace elements.

Sixty male FSW and 30 controls, from ten fuel stations at Al-Najaf City-Iraq, wereparticipated in the present study.

The AHR level in serum was measured using ELISAtechnique.

Determine the following metal ions Zn2+, Cu2+, Fe3+, Mg2+, Na+ and K+ level infilling station workers (FSW) and control group were measured spectrophotometrically byusing ready for use kits.

Serum Pb level was carried out using Atomic orptionspectroscopy.

The results serum concentration of AHR in FSW group revealed a significant increase(p<0.001) as compared with the control group.

No significant difference was noticed in AHRas compered in exposure ≥12years with exposure <12years in FWS.

Smoking has nosignificant correlation with other parameters.

Correlation study indicated a correlationbetween AHR and Age.

Serum concentration of Cu2+, Zn2+, K+ and Pb in FSW group revealeda significant increase (p<0.001) as compared with the control group.

While Fe3+, Na+ andMg2+ in FSW group revealed a significant decrease (p<0.001) as compared with the controlgroup.

Correlation study indicated a significant negative correlation between serum Pb andAHR while other trace elements showed no significant correlation with AHR in FSW group.

There is a significant negative correlation between serum Cu2+ with age while there issignificant increase correlation between Zn2+, Mg2+ and Pb with age in FWS group.