Verifying parentage and genetic variability among Arabian horse using ISSR markers
Joint Authors
Amin, Karam Abd al-Naim
al-Sayyid, Yasir S.
Tahsin, Raniya A.
Samak, Daliya H.
Nasr, Sharif M.
Source
Alexandria Journal of Veterinary Sciences
Issue
Vol. 59, Issue 1 (31 Oct. 2018), pp.125-133, 9 p.
Publisher
Alexandria University Faculty of Veterinary Medicine
Publication Date
2018-10-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The study aimed to verify parentage and genetic variability among twelve Arabian horses using five ISSR markers.
forty-five DNA loci, which extracted for the sire, mare and foal were amplified and replicated using the tested primers with an average of 9.0 loci per primer, and every marker is compared.
the total number of polymorphic alleles was 33 (73.33% polymorphism), with an average of 6.6 alleles per primer.
the markers (GAG)6G-ISSR (12 loci), (AGC)6C-ISSR (11 loci) and (AGC)6G-ISSR (9 loci) amplified the highest number of loci and possessed the highest PIC values indicating that they are the most promising for analysis of genetic variety and paternity in the Arabian horse.
the highest similarity and shortest genetic distance (Si=0.926, Gd=0.077) were between abeer and her daughter karma.
while, the lowest similarity and longest genetic distance (Si=0.353, Gd=1.041) between Sary and Farhan.
The UPGMA cluster analysis supported the genetic relatedness as expected among the 12 Arabian horses.
the dendrogram grouped the 12 Arabian horses according to their paternity, i.e.
pedigree levels, into three main clusters within a branched-off 0.439 genetic similarity.
American Psychological Association (APA)
Tahsin, Raniya A.& Amin, Karam Abd al-Naim& Samak, Daliya H.& al-Sayyid, Yasir S.& Nasr, Sharif M.. 2018. Verifying parentage and genetic variability among Arabian horse using ISSR markers. Alexandria Journal of Veterinary Sciences،Vol. 59, no. 1, pp.125-133.
https://search.emarefa.net/detail/BIM-1340874
Modern Language Association (MLA)
al-Sayyid, Yasir S.…[et al.]. Verifying parentage and genetic variability among Arabian horse using ISSR markers. Alexandria Journal of Veterinary Sciences Vol. 59, no. 1 (Oct. 2018), pp.125-133.
https://search.emarefa.net/detail/BIM-1340874
American Medical Association (AMA)
Tahsin, Raniya A.& Amin, Karam Abd al-Naim& Samak, Daliya H.& al-Sayyid, Yasir S.& Nasr, Sharif M.. Verifying parentage and genetic variability among Arabian horse using ISSR markers. Alexandria Journal of Veterinary Sciences. 2018. Vol. 59, no. 1, pp.125-133.
https://search.emarefa.net/detail/BIM-1340874
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 132-133
Record ID
BIM-1340874