Evaluation the sensitivity of vaginal lactobacilli to some physical conditions andchemical substances

Abstract EN

Six vaginal Lactobacillus strains (L.

acidophophillus, L.

crispatus, L.

jensenii, L.

fermentum, L.

tgasseri, L.

tcasei) were isolated from healthy women and tested against physical conditions and chemical substances, the outcomes appeared that all the six strains bacteriocin were completely destroyed by trypsin, proteinase k and pronase E treatments, while the activity of bacteriocins (except L.

gasseri bacteriocin) was maintained after treated with Chloroform, CaC03, Catalase and heating for 10 minutes at 60°C, 70°C, 80°C, 90°C, as well as, all six bacteriocins were stable to pH ranged between 4.5-7, but sensitive to pH 9, also the results clarified that all bacteriocins activity stable at 1% NaCl treatment, but this activity ended at 4% NaCl.

We can concluded that L.

acidophilus produced a more stable bacteriocin than the other isolated strains with promising inhibitory spectrum of antimicrobial activities against urogenital pathogens.