التوصيف الجزيئي لبعض أصناف و طرز المشمش في سورية IRAP باستخدام تقنية

Abstract EN

The genetic relationship was studied among six of apricot cultivars; i.e., Shukrbara,Tadmori, Baladi Mawardi, Baladi Habi, Wizri and Ajami in addition to five genotypes of Alklabi rootstock K1, K2, K3, K4 and K5 using Inter-Retrotransposon Amplified Polymorphism (IRAP) technique.

34 primer pairs of retrotransposons were used.

32 primer pairs showed high efficiency in characterizing the genetic diversity among the studied varieties and genotypes, with a polymorphism rate of 72.3%.

The IRAP technique showed highest degree of genetic similarity between the genotypes K4 and K5 (0.78) and the lowest degree between Baladi Mawardi and Wizri varieties (0.55), with an average genetic similarity of 0.67 among all the varieties and genotypes.

The cluster analysis grouped the studied apricot varieties and genotypes into two main clusters at 0.67 similarity degree and bootstrab value of 25%.

The first cluster contained six varieties distributed into two sub-clusters, the first sub-cluster contained two varieties: Baladi Habi and Wizri, while the second sub-cluster contained Tadmori and Shukrbara varieties along with K2 and K3 genotypes, and the second main cluster contained both genotypes K4 and K5.

The genotype K1 and Ajami variety separated away independently from both clusters and the genotype Baladi Mawardi remained distinguished from all studied materials.

The present study proved that the IRAP technique could produce unique bands that distinguish some local varieties and genotypes of apricot with high efficiency and reproducibility and also could estimate the genetic diversity among the varieties and genotypes.