Isolation, identification and immunological study of yeast from cattle and buffaloes in Basrah province, Iraq

الملخص EN

Two hundred samples taken from cattle and buffaloes (50 samples from each urine, blood, tracheal and vaginal swabs) were collected.

The study showed that C.albicans was the most common isolates from the total samples.

In cattle, it was isolated in a percentage 25 % out samples, followed by C.krusei 19 % % C.parapsilosis 11.5 %, C.10 % and C.rugosa 1.9, on the other hand other isolated yeasts were Cryptococcus neoformans 3.8 %, Geotricum candidum And Rhodotorula mucilaginous 1.9 %.

In buffaloes C.albicans was also found as a predominant isolates.

The total isolates of C.albicans was 34.9 % out of 14 % samples, followed by, C.tropicalis 22 %, C.krusei19.5 %, C.parapsilosis 9.4 % and C.rugosa 2.4 %.

Other yeasts were also identified such as Geotrichum candidum 4.9 % and Trichosporom cutaneum2.4 %.

C.rugosa was firstly isolated from urine samples taken from cattle and buffaloes and considered as newly recorded species from Iraq.

Statistical analysis showed significant difference (P < 0.001) between C.albicans isolation comparable with other yeasts in cattle and buffaloes.

Using Indirect Immunofluorescent antibody technique, the causative agent can be detected after 12 and 20 days after experimental infection of mice with C.albicans.

The result of using disc diffusion method for seven antifungal drugs showed significant differences P > 0.01 and P > 0.05 on susceptibility of the tested isolates toward antifungal drugs