Study of HL a class I and class II by (PCR-SSP)‎ and the role of salivary TNF-α in Iraqi patients with recurrent aphthous stomatitis

Abstract EN

Background : Until today, the etiology of recurrent aphthous stomatitis remains unknown, although hints of its etiologic basis lay on genetic susceptibility, infectious agents and alterations in immune mechanics.

Current study was established to shed light on the possible association of human leukocyte antigen class I and II alleles with recurrent aphthous stomatitis, and to investigate the possible alterations in salivary tumor necrosis factor-alpha level in patients and its relation with clinical types of disease. Subjects and Methods : The study included 55 subjects: 30 recurrent aphthous stomatitis patients and 25 apparently healthy subjects as control.

Polymerase chain reaction-specific sequence primers assay was conducted to assess human leukocyte antigen-typing whereas salivary tumor necrosis factor-alpha level was estimated by enzyme-linked immunosorbent assay. Results : The present study showed a significant association of HLA-Cw * 12 : 02 : 01-and DQB1 * 02 : 01 : 01- alleles with recurrent aphthous stomatitis as compared with healthy control, and there was significant low frequency of DQB1 * 05 : 01 : 01- allele in patients when compared with healthy control.

Furthermore, high frequency of DQB1 * 02 : 01 : 01- alleles was observed among patients with minor type of recurrent aphthous stomatitis when compared with healthy control.

Another interesting finding in this study was the significant elevation of salivary tumor necrosis factor-alpha level in patients than in healthy controls, as well as strong association of high salivary tumor necrosis factor-alpha level among patients who expressed DQB1 * 02 : 01 : 0-allell was observed. Conclusion : Cw * 12 : 02 : 01- and DQB1*02:01:01-alleles may played a role in the etiology of the disease, whereas DQB1 * 05 : 01 : 01-05 may confer protective effects against recurrent aphthous stomatitis.

Moreover; salivary tumor necrosis factor-alpha may play an important role in pathogenesis of disease, and may also have an important role in the search of new treatments for disease.