Genetic analysis and identification of RAPD markers linked to northern corn leaf blight disease resistance in a white maize population
Other Title(s)
التحليلات الوراثية و التعرف على الدلائل ال RAPD المرتبطة بالمقاومة لمرض لفحة أوراق الذرة الشامية في عشيرة الذرة البيضاء
Joint Authors
Milad, Sana Ibrahim Muhammad
Barakat, Muhammad Najib Rizq
al-Shafii, Adil M.
Khattab, Sabri Abd Allah
Source
Journal of King Abdul Aziz University : Meteorology, Environment and Arid land Agriculture Sciences
Issue
Vol. 20, Issue 1 (30 Jun. 2009), pp.45-61, 17 p.
Publisher
King Abdulaziz University Scientific Publishing Center
Publication Date
2009-06-30
Country of Publication
Saudi Arabia
No. of Pages
17
Main Subjects
Topics
Abstract AR
Northern corn leaf blight (NCLB) is an important disease occurring in maize producing areas.
Understanding the genetic nature of NCLB is regarded as a primer step in maize breeding programs.
This study used the resistant line Sids-63 (Sd-63) and the susceptible line Sids-7 (Sd-7) to develop F1 and F2 generations.
P1, P2, F1 and F2 were used to study the genetic nature and to detect RAPD markers associated with the NCLB resistance gene(s) using bulked segregant on analysis (BSA).
The genetic studies revealed that the inbred line, Sd63 recorded low disease severity (ranged from less than 5 % to 10 %) and the inbred line, Sd-7, recorded high disease severity (ranged from 26 % to more than 75 %).
F1 severity ranged from less than 5 % to 50 %.
The F2 severity ranged from less than 5 % to more than 75 %.
The observed ratio (number of plants with high : low disease severity) fitted the Mendelian ratio, 3 : 1, and suggested the operation of one gene pair in this cross.
The degrees of dominance for F1 (h1) and F2 (h2) gave significant negative values (–0.86 and –0.71 respectively).
The heritability value for the tested cross was 92.00 % and the estimated number of genes was 1.1, indicating that there was a difference in resistance genes between the two inbred lines.
Thirty-eight primers were screened to identify two RAPD markers; namely, Pr11180 and Pr11300 linked to NCLB susceptible genotypes.
Results from the two RAPD markers fitted the expected Mendalian ratio, 3 : 1, according to χ2-test .The regression analysis showed that the relationships between the two markers, Pr11180 and Pr11300, and the phenotype of the F2 individuals were significant and they recorded a determination coefficient (r2) = 0.54 and 0.60,for the two markers, respectively.
American Psychological Association (APA)
Barakat, Muhammad Najib Rizq& Milad, Sana Ibrahim Muhammad& al-Shafii, Adil M.& Khattab, Sabri Abd Allah. 2009. Genetic analysis and identification of RAPD markers linked to northern corn leaf blight disease resistance in a white maize population. Journal of King Abdul Aziz University : Meteorology, Environment and Arid land Agriculture Sciences،Vol. 20, no. 1, pp.45-61.
https://search.emarefa.net/detail/BIM-327520
Modern Language Association (MLA)
Barakat, Muhammad Najib Rizq…[et al.]. Genetic analysis and identification of RAPD markers linked to northern corn leaf blight disease resistance in a white maize population. Journal of King Abdul Aziz University : Meteorology, Environment and Arid land Agriculture Sciences Vol. 20, no. 1 (2009), pp.45-61.
https://search.emarefa.net/detail/BIM-327520
American Medical Association (AMA)
Barakat, Muhammad Najib Rizq& Milad, Sana Ibrahim Muhammad& al-Shafii, Adil M.& Khattab, Sabri Abd Allah. Genetic analysis and identification of RAPD markers linked to northern corn leaf blight disease resistance in a white maize population. Journal of King Abdul Aziz University : Meteorology, Environment and Arid land Agriculture Sciences. 2009. Vol. 20, no. 1, pp.45-61.
https://search.emarefa.net/detail/BIM-327520
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 57-59
Record ID
BIM-327520