Morphological, biochemical and molecular characterization of ten rhizobial bacteria isolates

Abstract EN

This study was done in biotechnology laboratories in the national center of organic farming / ministry of agriculture where ten of Rhizobial isolates and strain studied were either local isolate from chickpea root nodules or non-local (Syrian and Turkish) obtained from ICARDA.

These isolates were identified and characterized on the basis of colonies morphology and biochemical tests including gram staining, catalase and oxidase tests.

The Genetic diversity among the isolates was assessed by RAPD (Randum Amplified Polynorphic DNA)-PCR (Polynerase Chain Reaction) finger printing by using five primers.

The RAPD result showed high ability to detect genetic polymorphism in Rhizobia and have the ability to generated unique bands (marker) especially in Shiekhan 3(10) bands Mosle(8)bands isolates that isolated from chickpea plants.