Effect of plasmid size on the transformation of E. coli HB101
Other Title(s)
تأثير حجم البلازميد على تحول بكتريا القولون E. coli HB101
Author
al-Faysal, Abd al-Husayn Muwayt
Source
Iraqi Journal of Biotechnology
Issue
Vol. 5, Issue 1 (30 Jun. 2006), pp.123-131, 9 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2006-06-30
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Abstract AR
Plasmids are one of the most common vectors used in cloning.
Many factors have been repotted to affect the bacterial transformation by plasmids.
This paper an attempt to find the effect of plasmid size on the transformation efficiency of E.coli HB101.
Our results showed that the small sized Plasmids more effective in transforming of the bacteria E.coli HBCOL was) (4.1-4.8kb than the large ones (5.7-6kb).
The transformation efficiency with the small sized plasmids reaches between 6×610-7×610 comparing with 4.1 ×105- 4.6×105 of the large sized plasmid.
We conclude that the small sized plasmids are more suitable to use in cloning than large sized plasmids.
Abstract EN
Plasmids are one of the most common vectors used in cloning.
Many factors have been repotted to affect the bacterial transformation by plasmids.
This paper an attempt to find the effect of plasmid size on the transformation efficiency of E.coli HB101.
Our results showed that the small sized Plasmids more effective in transforming of the bacteria E.coli HBCOL was) (4.1-4.8kb than the large ones (5.7-6kb).
The transformation efficiency with the small sized plasmids reaches between 6 × 610-7 × 610 comparing with 4.
1 ×105-4.
6 × 105 of the large sized plasmid.
We conclude that the small sized plasmids are more suitable to use in cloning than large sized plasmids.
American Psychological Association (APA)
al-Faysal, Abd al-Husayn Muwayt. 2006. Effect of plasmid size on the transformation of E. coli HB101. Iraqi Journal of Biotechnology،Vol. 5, no. 1, pp.123-131.
https://search.emarefa.net/detail/BIM-350475
Modern Language Association (MLA)
al-Faysal, Abd al-Husayn Muwayt. Effect of plasmid size on the transformation of E. coli HB101. Iraqi Journal of Biotechnology Vol. 5, no. 1 (Jun. 2006), pp.123-131.
https://search.emarefa.net/detail/BIM-350475
American Medical Association (AMA)
al-Faysal, Abd al-Husayn Muwayt. Effect of plasmid size on the transformation of E. coli HB101. Iraqi Journal of Biotechnology. 2006. Vol. 5, no. 1, pp.123-131.
https://search.emarefa.net/detail/BIM-350475
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 129-131
Record ID
BIM-350475
