العلاقة المصلية بين فايتوبلازما مكنسة الساحرة على الطرطيع (Suaeda baccata Forssk)‎ و تلك التي تصيب بعض المحاصيل الاقتصادية و نباتات الأدغال-الأعشاب في العراق

Abstract EN

This study was earned out to determine the serological relationship between suaeda (Suaeda baccatum) Witches’ broom phytoplasma (SWB) and those infecting several other crops and weeds in Iraq.

Partial purification of suaeda phytoplasma was achieved by centrifugation through pcrcoll gradient (15-50%) at 20000g for 20 min.

Antiserum for this phytoplasma was obtained within 6 weeks following 12 intramuscular and subcutaneous injections in a rabbit The rabbit was bled two weeks after the last injection.

Results of double immuno diffusion test showed a positive reaction between SWB-AS and its antigen or extracts from infected plants, in PBS buffer, in an agarose medium containing sodium dodccyl sulfate (SDS).

These results indicated the possibility of utilizing this technique for phytoplasma detection.

A cross-reaction was noticed between SWB-AS and extracts from tomato plants showing Witchcs’ broom and big bud symptoms, mulbery fasciation, wild lettuce phyllody and yellow thistle which indicated that these phytoplasmas arc serologically related No reaction was observed between SWB-AS and extracts from phytoplasma-int'ected potato and sesame, indicating that such phytoplasmas represent a different serogroup Key words: suaeda, phytoplasma.

Witches’ broom