![](/images/graphics-bg.png)
Effect of exogenous interleukin-10 on carbon tetrachloride CCL4-induced hepatic fibrosis in rats
Joint Authors
Hasan, Jamal Muhammad Abd al-Rahman
Abd al-Hamid, Ala al-Din Sad
Nasir al-Din, Wail A.
Kamil, Amr A.
Source
Suez Canal University Medical Journal
Issue
Vol. 16, Issue 1 (30 Jun. 2013), pp.29-36, 8 p.
Publisher
Suez Canal University Faculty of Medicine
Publication Date
2013-06-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Topics
Abstract EN
Background : Transforming growth factor β1 (TGF-β1) is one of the strongest profibrotic cytokines and TGF-β1 / Smad signaling is the cardinal signal transduction pathway involved in fibrosis.
Previ-ous studies indicated that exogenous IL-10 down regulates collagen type I.
It also exerts antifibrogenic effect by down regulating profibrogenic cytokines such as (TGF-β1).
All these stud-ies indicate that IL-10 might become a new therapeutic target.
Aim : To investigate the potential therapeutic effect of exogenous interleukin-10 on reversing the well-established hepatic fibrosis of CCl4 administration in experimental rats.
Materials and Methods : Albino rats were divided into four groups (27 rats each) : group A (control), group B1 (CCl4-treated), group B2 (spontaneous re-covery, SR) and group B3 (IL-10-treated).
Rats' liver tissue was stained with i) hematoxylin and eosin (H & E) and, ii) Masson’s trichrome stains for evaluation of the histological activity index (HAI).
Serum TGF-β1 was determined by ELISA.
Results : No inflammation was found in the control group, however, marked inflammation (grade 3) was observed in CCl4-induced fibrosis group while moderate inflammation (grade 2) was observed in SR group.
Meanwhile the administration of IL-10 in group D resulted in a marked decrease in the grading of inflammation (grade 1) com-pared to CCl4-induced fibrosis group.
No fibrosis was observed in the control group, however, a marked fibrosis reaching to cirrhosis (stages 3&4) was observed in CCl4-induced fibrosis group.
The degree of fibrosis showed a mild decrease (stage 3) in SR group.
Meanwhile the administra-tion of IL-10 in group D resulted in a marked decrease in the stage of fibrosis to (stage 1).
Serum TGF-β1 concentration dramatically increased in CCl4-induced fibrosis group compared to the con-trol group.
It also increased in SR group, but lesser than CCl4 treated group while it was signifi-cantly reduced in the IL-10 treated group.
Conclusions : Our results provide evidence towards the potential effect of IL-10 as anti-TGF-β1 that could lead to a reduction of hepatic fibrosis.
American Psychological Association (APA)
Nasir al-Din, Wail A.& Hasan, Jamal Muhammad Abd al-Rahman& Abd al-Hamid, Ala al-Din Sad& Kamil, Amr A.. 2013. Effect of exogenous interleukin-10 on carbon tetrachloride CCL4-induced hepatic fibrosis in rats. Suez Canal University Medical Journal،Vol. 16, no. 1, pp.29-36.
https://search.emarefa.net/detail/BIM-380588
Modern Language Association (MLA)
Abd al-Hamid, Ala al-Din Sad…[et al.]. Effect of exogenous interleukin-10 on carbon tetrachloride CCL4-induced hepatic fibrosis in rats. Suez Canal University Medical Journal Vol. 16, no. 1 (2013), pp.29-36.
https://search.emarefa.net/detail/BIM-380588
American Medical Association (AMA)
Nasir al-Din, Wail A.& Hasan, Jamal Muhammad Abd al-Rahman& Abd al-Hamid, Ala al-Din Sad& Kamil, Amr A.. Effect of exogenous interleukin-10 on carbon tetrachloride CCL4-induced hepatic fibrosis in rats. Suez Canal University Medical Journal. 2013. Vol. 16, no. 1, pp.29-36.
https://search.emarefa.net/detail/BIM-380588
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 34-36
Record ID
BIM-380588