التغيرات الجزيئية بين سلالات من الذرة الصفراء

الملخص EN

This study was carried out at the Agricultural Research Laboratories during 2013.

The objective was to investigate genetic diversity among 10 maize inbred lines.

The random amplified polymorphic DNA (RAPD) based on PCR with 10 primers.

RAPD DNA markers were used to evaluate trends in genetic diversity among 10 0f inbred line .All of the RAPD primers used for initial screening were found to be polymorphic.

A total of 108 DNA fragments were generated by 11 random decamer primers with an average of 10.7 per primer ranged.

The number of amplified fragments produced per primer ranged from 5 for OPAV-03 primer to 17 for OPAW-10, from with molecular size ranged from 160 bp to 1800 bp.

The total number of polymorphic fragment and the percentage of polymorphism were (74, 62.7 %) respectively.

Maximum level of polymorphism was 80% 0bserved for the primer OPAK -15 while Primer OPAW -11 showed the lowest percentage of polymorphism.

Maximum level of primer efficiency and ability distinction was 14.1%, 17.1% respectively.

Based on the bivariate (1 -0) data and genetic similarity with the use of UPGMA cluster method, the dendrogram separated the studied populations in to A and B.

Cluster analysis which compared between inbred lines in to dendrogram.

Showed BK104 high genetic diversity was high genetic diversity between BK104 and BK164.

Genetic similarities computed by Nei and Li,s similarity coefficient revealed that the highest estimate (0.839) was observed between inbred line BK164, BK147.

While, the lowest genetic similarity between inbred line BK104, BK128 and BK104, BK164 (0.377, 0.396) respectively.

Results indicated that RAPD were highly efficient in delecting the purity and genetic relationship among maize inbred lines.