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Investigation of streptococcus mutans isolated from dental caries patients
Joint Authors
Flayyih, Isra Abd al-Amir
al-Ammar, Mahdi Hasan
Source
al-Kufa University Journal for Biology
Issue
Vol. 7, Issue 1 (30 Jun. 2015)13 p.
Publisher
University of Kufa Faculty of Science Department of live Sciences
Publication Date
2015-06-30
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Topics
Abstract AR
Introduction: The clinical specimens (100) were collected from patients suffering from dental plague infectious during the period from October ( 2012 to March 2013) in Najaf City .
Methodology: Streptococcus mutans isolates were detected by cultural and biochemical tests as well as API 20strep , then confirm the GTFB gene by PCR technique .
Result: Streptococcus mutans were recorded (40%) among Mutans Streptococcus from total isolates .
The virulence factors of bacteria were detected and the results showed that all isolates were produced glucan,biofilm and Dextranase 40(100%) and varied produce heamolysin 30(75%) .
Also, 20-25(50-62.5%) of Streptococcus mutans has the colonization factor antigen III, ,I respectively and 15 (37.5%) of isolates are mutacin production.Finally, Molecular study by PCR technique was used for detection gtfB gene, it was found the gtfB gene predominance in 22 (55%) of clinical isolates .
Conclusion: The study showed that Streptococcus mutans have gtfB gene which responsible for the pathogenicity of Streptococcus mutans .
Abstract EN
Introduction: The clinical specimens (100) were collected from patients suffering from dental plague infectious during the period from October ( 2012 to March 2013) in Najaf City .
Methodology: Streptococcus mutans isolates were detected by cultural and biochemical tests as well as API 20strep , then confirm the GTFB gene by PCR technique .
Result: Streptococcus mutans were recorded (40%) among Mutans Streptococcus from total isolates .
The virulence factors of bacteria were detected and the results showed that all isolates were produced glucan,biofilm and Dextranase 40(100%) and varied produce heamolysin 30(75%) .
Also, 20-25(50-62.5%) of Streptococcus mutans has the colonization factor antigen III, ,I respectively and 15 (37.5%) of isolates are mutacin production.Finally, Molecular study by PCR technique was used for detection gtfB gene, it was found the gtfB gene predominance in 22 (55%) of clinical isolates .
Conclusion: The study showed that Streptococcus mutans have gtfB gene which responsible for the pathogenicity of Streptococcus mutans .
American Psychological Association (APA)
Flayyih, Isra Abd al-Amir& al-Ammar, Mahdi Hasan. 2015. Investigation of streptococcus mutans isolated from dental caries patients. al-Kufa University Journal for Biology،Vol. 7, no. 1.
https://search.emarefa.net/detail/BIM-594205
Modern Language Association (MLA)
Flayyih, Isra Abd al-Amir& al-Ammar, Mahdi Hasan. Investigation of streptococcus mutans isolated from dental caries patients. al-Kufa University Journal for Biology Vol. 7, no. 1 (2015).
https://search.emarefa.net/detail/BIM-594205
American Medical Association (AMA)
Flayyih, Isra Abd al-Amir& al-Ammar, Mahdi Hasan. Investigation of streptococcus mutans isolated from dental caries patients. al-Kufa University Journal for Biology. 2015. Vol. 7, no. 1.
https://search.emarefa.net/detail/BIM-594205
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 10-13
Record ID
BIM-594205