Detection of codon 1213 g.6262G>A mutation of H-ras gene in Iraqi bladder carcinoma patients

Abstract EN

DNA was extracted from blood and urine samples from 45 patients with bladder carcinoma (age 20-87 years) in addition to samples from 25 apparently healthy persons as controls.

Restriction fragment length polymorphism (RFLP) analysis was performed to determine genotypes of the H-ras codons12,13 using Msp1 enzyme.

The healthy results showed that two fragments (165 bp and 55 bp) were produce from the digestion with the enzyme for H-ras codon 12/13.

These results indicated that the PCR amplified region of the codon 12/13 has one restriction site for the enzyme Msp1.

The molecular analysis of the patient samples revealed that among 45 patients included in this study, 28 patients (62.2 %) were with normal pattern (165 bp and 55 bp) and 17 patients (37.8 %) were homozygous mutants (g.6262G>A).

The frequency of g.6262 C>G mutation in patients was significantly higher than in apparently healthy subjects (37.3 % versus 0 %, OR = 0.033 ; X2 = 0.966*, P < 0.01).