A study of FLT ITD mutations in cytogenetically normal Iraqi acute myeloid leukemia patients
Other Title(s)
دراسة الطفرات الوراثية في جين FLT ITD لبعض مرضى ابيضاض الدم الحاد العراقيين ذوي الخلايا الطبيعية
Joint Authors
al-Faysal, Abd al-Husayn Muwayt
Ramadan, Rihab Subhi
Abbas, Muhammad Salim
Muhammad, Samarah Kazim
Source
Iraqi Journal of Biotechnology
Issue
Vol. 13, Issue 2(s) (31 Dec. 2014), pp.152-160, 9 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2014-12-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
- Biology
- Patients
- Embryology
- Genetics
- Chromosomes
- Biological adaptation
- Iraq
- Acute myeloid leukemia
- Acute leukemia
Abstract EN
The present study was designed to shed light on the molecular effects caused by acute myeloid leukemia (AML) pathogenesis in three cases before, during and after treatment with chemotherapy (in vitro) in lymphocytes.
It was also aimed to investigate FLT3/ITD point mutations in cytogenetically normal-AML region 100-300 bp compared to healthy control.
The study comprised of 30 AML Iraqi patients and their ages ranged between 2.5-81 years.
It included 12 females and 18 males compared with 26 healthy controls.
Results revealed that the extracted DNA from 30 AML patients and amplified by PCR to obtain FLT3/ITD gene from exon 11 to 12 showed larger bands (470 and 460) bp in 2 patients when compared to wild type (330) bp.
Among six patients, three of them displayed point mutations of deletion and substitution, while the others were normal since no mutations were detected.
The percentages of mutation types were substitution 77.8% and deletion 22.2%.
The types of mutations were missense mutations as (55.54 %), deletion (22.22 %) and nonsense (22.22 %).
American Psychological Association (APA)
Muhammad, Samarah Kazim& al-Faysal, Abd al-Husayn Muwayt& Abbas, Muhammad Salim& Ramadan, Rihab Subhi. 2014. A study of FLT ITD mutations in cytogenetically normal Iraqi acute myeloid leukemia patients. Iraqi Journal of Biotechnology،Vol. 13, no. 2(s), pp.152-160.
https://search.emarefa.net/detail/BIM-643496
Modern Language Association (MLA)
al-Faysal, Abd al-Husayn Muwayt…[et al.]. A study of FLT ITD mutations in cytogenetically normal Iraqi acute myeloid leukemia patients. Iraqi Journal of Biotechnology Vol. 13, no. 2 (2014), pp.152-160.
https://search.emarefa.net/detail/BIM-643496
American Medical Association (AMA)
Muhammad, Samarah Kazim& al-Faysal, Abd al-Husayn Muwayt& Abbas, Muhammad Salim& Ramadan, Rihab Subhi. A study of FLT ITD mutations in cytogenetically normal Iraqi acute myeloid leukemia patients. Iraqi Journal of Biotechnology. 2014. Vol. 13, no. 2(s), pp.152-160.
https://search.emarefa.net/detail/BIM-643496
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 159-160
Record ID
BIM-643496