تقصي انتشار بعض الفيروسات على البطاطا البطاطس في سورية، و الكشف عن السلالات الرئيسة لفيروس البطاطا البطاطس واي مصلياً سيرولوجيا

Abstract EN

A total of 428 symptomatic potato compound leaf samples (each represented 5 plants) were collected from 6 Syrian governorates and tested for the presence of Potato virus Y (PVY), Cucumber mosaic virus(CMV), Alfalfa mosaic virus (AMV) and Tomato spotted wilt virus (TSWV) during 2008 by DAS-ELISA using PAbs.

Results showed the diversity in their incidence, according to cropping season and location.

Tested viruses incidence was the highest in spring cropping season, compared with summer and autumn seasons.

PVY incidence was the highest on potato plants in all cropping seasons and governorates, with the exception of Daraa province, where the incidence rates of viruses PVY, CMV, and AMV were equal on plants of the spring cropping season, whereas CMV incidence declined in comparison with PVY and AMV incidences in the autumn cropping season.

The overall rate of infection in tested compound samples was 6.4%, 3.8, 3.6 and 0.7% for PVY, AMV, CMV and TSWV, respectively.

The percentage of selected individual samples, which reacted positively with PVY-PAbs was 48.6% in 929 samples, collected from potato plants during the spring cropping season in all surveyed governorates, during 2009.

Three hundred and sixty PVY isolates were serologically characterized by DAS-ELISA using four antisera, three of them to reveal the main strains individually, and the fourth is a cocktail MAbs designed to detect any/all previous strains.

The results showed that the tested isolates could be placed in 4 different groups.

The strains PYVO, PYVN and PYVC accounted for 45.8, 33.3 and 4.2% of the isolates, respectively.

Meanwhile, 1.4% of the tested isolates did not react with any of the MAbs used, which indicates the possible existence of various other strains/structures of the virus in Syria.

15.3% of the isolates reacted with more than one MAb, 92.7% of them reacted positively with both PVYO and PVYN specific MAbs.

PVYO was the most frequent in samples from all governorates, excluding Homs, where PVYN was the most frequent, while PVYC was recorded only in Homs and Hama governorates.

Generally, PVYO and PVYC induced systemic mild mosaic, whereas PVYN induced mottle, vein clearing and sometimes necrosis, and leaf distortion based on the indicator plant used.