Evaluating the bacterial culture technique by polymerase chain reaction for the diagnosis of brucella abortus in milk of cows suspected with brucellosis
Other Title(s)
استعمال اختبار تفاعل انزيم البلمرة المتسلسل في تقييم طريقة الزرع الجرثومي المستعملة في تشخيص البروسيلا المجهضة في حليب الأبقار المشتبه بإصابتها بداء البروسيلات
Joint Authors
Numi, Bashshar Sadiq
Jafar, Nihad Abd al-Husayn
Source
Iraqi Journal of Veterinary Medicine
Issue
Vol. 40, Issue 1 (30 Jun. 2016), pp.5-8, 4 p.
Publisher
University of Baghdad College of Veterinary Medicine
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
4
Main Subjects
Topics
Abstract EN
The aim of this study is to determine the sensitivity of bacterial culture technique in the detection of Brucella abortus in milk samples of aborted cows.
Sixty samples of milk were collected from aborted cows during a period which did not exceed two months after the abortion.
All of them were positive for rose bengal test.
Results showed that Brucella abortus was isolated from 7 out of 60 (11.6%) from the milk of aborted cows, while PCR test showed that 32 out of 60 (53.3%) milk sample contained Brucella abortus.
The specificity of culture techniques was 10%, but its sensitivity was only 21.8%.
Beside the cautions in dealing with live Brucella abortus (as culture), it is also less sensitive than PCR, though it is better to use PCR technique in the diagnosis of brucellosis in aborted cows milk.
American Psychological Association (APA)
Numi, Bashshar Sadiq& Jafar, Nihad Abd al-Husayn. 2016. Evaluating the bacterial culture technique by polymerase chain reaction for the diagnosis of brucella abortus in milk of cows suspected with brucellosis. Iraqi Journal of Veterinary Medicine،Vol. 40, no. 1, pp.5-8.
https://search.emarefa.net/detail/BIM-701322
Modern Language Association (MLA)
Numi, Bashshar Sadiq& Jafar, Nihad Abd al-Husayn. Evaluating the bacterial culture technique by polymerase chain reaction for the diagnosis of brucella abortus in milk of cows suspected with brucellosis. Iraqi Journal of Veterinary Medicine Vol. 40, no. 1 (2016), pp.5-8.
https://search.emarefa.net/detail/BIM-701322
American Medical Association (AMA)
Numi, Bashshar Sadiq& Jafar, Nihad Abd al-Husayn. Evaluating the bacterial culture technique by polymerase chain reaction for the diagnosis of brucella abortus in milk of cows suspected with brucellosis. Iraqi Journal of Veterinary Medicine. 2016. Vol. 40, no. 1, pp.5-8.
https://search.emarefa.net/detail/BIM-701322
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 7-8
Record ID
BIM-701322