Relationship between hepatic copper concentration and liver enzymes activity levels in buffaloes

Abstract EN

This study was conducted on 35 buffalo calves, which were randomly collected from a buffalo farm.

All calves were classified according to the determination of hepatic and serum Cu concentration.

(10) of them considered as a control group, while the rest ( 25) considered as cu accumulated group.

All calves were slaughtered in Bani Adi slaughter house (Assiut, Egypt).

Blood and liver samples were collected during ordinary slaughter of the animals.

Serum samples used for determination of serum copper concentration, ceruloplasmin (CP) activity, and hepatic enzyme activities (AST and GGT).Cu concentration was determined in the liver samples and feed stuffs.

Results showed that the mean value of both AST and GGT enzymes activity were significant (p<0.01) and (p< 0.05) increased in the cu accumulated group than the control one.

The mean values of serum cu and hepatic cu were significant (p<0.01) increase in the cu accumulated group than the control group and also for molybdenum while for cereloplasmin was non-significant.

The mean value of cu concentration in barseem, wheat straw and concentrates were evaluated.

Significant correlation between hepatic Cu accumulation, serum AST activity and serum GGT activity were recorded in this study.

Analysis of Cu content in the liver with determination of both AST and GGT enzymes activity is probably the best diagnostic tool currently available for assessing the risk of increase cu accumulation