Some epidemiological features of BCoVs infection in al Qadisiya Province by using real time-qPCR technique
Other Title(s)
دراسة بعض الصفات الوبائية للفايروس التاجي البقري في محافظة القادسية باستخدام خطوة واحدة في الوقت الحقيقي الكمي للفلورة-تفاعل البلمرة العاكس الناسخ
Joint Authors
al-Hamdawi, Khatam Qayid Myia
al-Rawdan, Muhsin Abd Nimah
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 13, Issue 2 (31 Dec. 2014), pp.14-19, 6 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2014-12-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
This study was carried out to evaluate some epidemiological features of Bovine Coronavirus infection by using one-step real-time fluorogenic quantitative reverse transcription polymerase chain reaction (RT-qPCR) assay based on SYBR Green I dye in detection.
Coronaviruses detected by the same neocleocapsid (N) gene primers under 98% similarity with HECV-4408 (human enteric Coronavirus) in children according to NCBI with product size 124bp.
285 fecal samples has been examined by routine methods against pathogenic bacteria in the intestines (E.coli, Salmonella Spp.) and Cryptosporidium parvum, the samples positive for the parasite submitted to molecular testing because they may be mixed with coronavirus infections.
100 samples were screened for the presence of BCoVs antigens by using a immunochromatographic rapid test as a field fast test.
Where 44% of samples showed positivity to BCoVs, out of 50 samples submitted to quantitative reverse transcription (RT-qPCR) assay.
Out of 50 – 31 samples had been positive.
We found, that distribution of BCoVs was significantly higher in rural areas 33.3%-87.5% as compare to cities 71.4%-75%, the infection in males reach to75% vas 53.5% in females, high infection rate 62.9% in < 1- 4 months age as compare with > 1 month age 62.9%.
The results of infection rate showed high percentage during February 77.7% while the percentage on (January, December, March, and April) was (66.6%, 66.1%, 54.5% and 50%) respectively.
American Psychological Association (APA)
al-Hamdawi, Khatam Qayid Myia& al-Rawdan, Muhsin Abd Nimah. 2014. Some epidemiological features of BCoVs infection in al Qadisiya Province by using real time-qPCR technique. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 13, no. 2, pp.14-19.
https://search.emarefa.net/detail/BIM-743365
Modern Language Association (MLA)
al-Hamdawi, Khatam Qayid Myia& al-Rawdan, Muhsin Abd Nimah. Some epidemiological features of BCoVs infection in al Qadisiya Province by using real time-qPCR technique. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 13, no. 2 (2014), pp.14-19.
https://search.emarefa.net/detail/BIM-743365
American Medical Association (AMA)
al-Hamdawi, Khatam Qayid Myia& al-Rawdan, Muhsin Abd Nimah. Some epidemiological features of BCoVs infection in al Qadisiya Province by using real time-qPCR technique. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2014. Vol. 13, no. 2, pp.14-19.
https://search.emarefa.net/detail/BIM-743365
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 18-19
Record ID
BIM-743365