دراسة إنتاجية حامض الكلوتاميك من بكتريا Bacillus subtilis EN3 معزولة من التربة
Other Title(s)
Study productivity of glutamic acid by Bacillus subtilis EN3 isolated from the soil
Joint Authors
الراجحي، شيماء حسين
العطار، إيمان جابر جاسم
الجنابي، نضال محمد صالح
Source
مجلة ابن الهيثم للعلوم الصرفة و التطبيقية
Issue
Vol. 29, Issue 2 (31 Aug. 2016), pp.340-356, 17 p.
Publisher
University of Baghdad College of Education for Pure Science / Ibn al-Haitham
Publication Date
2016-08-31
Country of Publication
Iraq
No. of Pages
17
Main Subjects
Topics
Abstract EN
The genus Bacillus bacterium isolated from soil and tests their ability to produce glutamic acid, the bacteria are diagnosed by traditional methods and VITEK-2 system.
And we studied the optimal conditions for production and try to improve the productivity of the isolation by manipulating the components of the production medium and of the circumstances environmental of production medium.
There is 43 isolates of the 70 bacterial isolates belonging to the genus Bacillus depending on phenotypic characteristics of the colonies and microscopic characteristics and found that 13 isolated revert to species B.subtilis depending on physiological and biochemical characteristics.
As well as on microscopic and biochemical tests, 13 isolates were subjected to the diagnostic by VITEK-2 system.
The results showed that EN5 and EN3 diagnosed as B.subtilis as well as a commercial isolate A1 was also diagnosed as B.subtilis was selected EN3 isolation, as highest productivity (2 mg/ml).then optimal conditions was studied to produce glutamic acid by using the semi synthesis medium contained waste of extract dates (DE) as best Carbone source at 10% and use of ammonium nitrate as the best source of nitrogen at 2% from the production medium, and achieved better productivity of glutamic acid amounted to 7.2 mg/ml, at the temperature 34°C, pH initial 6.5, ventilation and agitation increase concentration of glutamic acid product when the proportion of medium size to the size of the flask 5:1 in the shaker incubator speeds of 180 rpm, for incubated period of 72 hours, were obtained, improvement rate was amounted to 360% from what was produced wild isolation.
American Psychological Association (APA)
العطار، إيمان جابر جاسم والجنابي، نضال محمد صالح والراجحي، شيماء حسين. 2016. دراسة إنتاجية حامض الكلوتاميك من بكتريا Bacillus subtilis EN3 معزولة من التربة. مجلة ابن الهيثم للعلوم الصرفة و التطبيقية،مج. 29، ع. 2، ص ص. 340-356.
https://search.emarefa.net/detail/BIM-746007
Modern Language Association (MLA)
الجنابي، نضال محمد صالح....[و آخرون]. دراسة إنتاجية حامض الكلوتاميك من بكتريا Bacillus subtilis EN3 معزولة من التربة. مجلة ابن الهيثم للعلوم الصرفة و التطبيقية مج. 29، ع. 2 (2016)، ص ص. 340-356.
https://search.emarefa.net/detail/BIM-746007
American Medical Association (AMA)
العطار، إيمان جابر جاسم والجنابي، نضال محمد صالح والراجحي، شيماء حسين. دراسة إنتاجية حامض الكلوتاميك من بكتريا Bacillus subtilis EN3 معزولة من التربة. مجلة ابن الهيثم للعلوم الصرفة و التطبيقية. 2016. مج. 29، ع. 2، ص ص. 340-356.
https://search.emarefa.net/detail/BIM-746007
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن ملاحق : ص. 349-355
Record ID
BIM-746007