Evaluate the performance of field and productive for the seedlings potatoes resulting from in vitro preservation

الملخص EN

Research's experiments were carried out in plant tissue culture labs.

Date Palm Research Unit, College of Agriculture, University of Baghdad, Iraq from Jun 2012 to July 2013.

Experiments included adding Cytokinen represented by kinetin (0, 3, 5 and 7 mg/L) to MS media with 80g/L sucrose for microtuber initiation and mannitol (0, 10, 20 and 40 g/1) and ABA (0, 2.0, 4.0 mg/l) to MS media with 30g/L sucrose for three potato cultivars Emma, Sante, and Arnova.

For microtuber initiation, after 8 weeks it’s clear that the best concentration was 7mg/L for all recipes under study (average of microtubers number/plant, average weight of the microtubers/plant (g), average diameter of the microtubers (mm), percentage of dry matter of microtubers, percentage of starch in microtubers, and percentage of protein in microtubers ) but the cultivars were different, microtubers were harvest and preserved at 4°C for three periods 2, 4, and 6 months (after placed in glass Jars covered with cotton) after those periods microtubers cultured in plastic pot to growth.

For the slow growth conservation using mannitol and ABA the explant also preserved for 2, 4, and 6 months and then transferred to regeneration media (MS free hormones) and maintain for four weeks then transferred to acclimation stage for four weeks also, 40g/I mannitol was the best treatment for acclimation (data not shown) then those plant transferred to greenhouse which supplemented with cooling (fan and pad) system, until plant reaches the production stage.

Before the shoots dry and yellowing, vegetative growth parameters were measured.

Tuber harvesting has been done in 1-6-2013 and yield parameters were calculated.

The best method for conservation to production tubers was slow growth preservation compared to microtuber preservation for all traits under study.