Using of epididymal sperms of local Iraqi bucks for in vitro fertilization

Abstract EN

Sixty female genital organs of black local Iraqi goats were collected from the abattoir during the breeding season, and transported in a normal saline in a cool box within 30 minutes of slaughter to the Theriogenology lab.

The oocytes were collected either by aspiration or slicing and graded on the basis of the nature of cumulus cells and homogeneity of cytoplasm.

The medium used for maturation and for IVF were TCM-199, TALP and MEM.

Caudal epididymal sperms were used for IVF.

Results of the present study showed a high level of maturation and fertilization rates observed in TCM-199, followed by TALP and MEM medium.

Thus it could be concluded that there is a possibility of getting a successful IVF in Iraqi goats by using epididymal sperms.