Detecting mRNA expression of LUNX gene by RT-PCR in lung cancer specimens of Iraqi patients
Joint Authors
al-Faysal, Abd al-Husayn Muwayt
al-Sadi, Muhannad K.
al-Fartusi, Abd Allah M.
al-Ulwan, Nada Abd al-Sahib
Source
Iraqi Journal of Biotechnology
Issue
Vol. 16, Issue 1 (31 Mar. 2017), pp.29-39, 11 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2017-03-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Topics
Abstract EN
This study was conducted to evaluate the clinical significance of mRNA expression of lung-specific X protein (LUNX) in specimens obtained from Iraqi patients.
In the present study, whole blood was isolated from 140 individuals distributed into four groups as follows; Group 1: 40 Samples from smoker patients affected by lung cancer, Group 2: 40 Samples from non-smoker patients affected by lung cancer, Group 3: 30 Samples from smokers apparently healthy individuals and Group 4: 30 Samples from nonsmokers apparently healthy individuals.
The messenger RNA (mRNA) expression levels of LUNX in the peripheral blood were analyzed using reverse transcription‑polymerase chain reaction (RT‑PCR).
The expressions of LUNX mRNA in these groups were 11.0 folds of that in group 1 compared with group 4, 10 folds of gene Expression in group 2 compared with group 4 and 3.0 folds of gene expression in group 3 compared with group 4, using GAPDH as Housekeeping Gene.
Statistical results showed that there was a high correlation between the LUNX gene expression and lung cancer; where the correlation coefficient was 0.879 and a low correlation between LUNX gene expression and smoking; where the correlation coefficient was 0.446.
The results indicate that the mRNA expression of LUNX could yield a first picture to the early detection of lung cancer in the peripheral blood and thus is of significant clinical value for the diagnosis of lung cancer.
American Psychological Association (APA)
al-Sadi, Muhannad K.& al-Faysal, Abd al-Husayn Muwayt& al-Ulwan, Nada Abd al-Sahib& al-Fartusi, Abd Allah M.. 2017. Detecting mRNA expression of LUNX gene by RT-PCR in lung cancer specimens of Iraqi patients. Iraqi Journal of Biotechnology،Vol. 16, no. 1, pp.29-39.
https://search.emarefa.net/detail/BIM-769127
Modern Language Association (MLA)
al-Fartusi, Abd Allah M.…[et al.]. Detecting mRNA expression of LUNX gene by RT-PCR in lung cancer specimens of Iraqi patients. Iraqi Journal of Biotechnology Vol. 16, no. 1 (2017), pp.29-39.
https://search.emarefa.net/detail/BIM-769127
American Medical Association (AMA)
al-Sadi, Muhannad K.& al-Faysal, Abd al-Husayn Muwayt& al-Ulwan, Nada Abd al-Sahib& al-Fartusi, Abd Allah M.. Detecting mRNA expression of LUNX gene by RT-PCR in lung cancer specimens of Iraqi patients. Iraqi Journal of Biotechnology. 2017. Vol. 16, no. 1, pp.29-39.
https://search.emarefa.net/detail/BIM-769127
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 38-39
Record ID
BIM-769127