Optimization and characterization growth conditions of lipase-producing bacteria from oil contaminated soil and sewage
Joint Authors
Muhsin, Yusra M. B.
al-Kaki, Sana Nuri Husayn
Baqir, Batul Abd al-Amir
Hamid, Inam
Ubayd, Khitam Ali
al-Kabi, Sadiq Abd al-Rida Qatia
Source
Iraqi Journal of Biotechnology
Issue
Vol. 15, Issue 3 (31 Dec. 2016), pp.65-70, 6 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract EN
Ten samples of oil contaminated sewage and soil were collected from generators locations and car repair locations in Baghdad, Iraq for isolation of lipase- producing bacteria.
Enriched selective media technique was used for isolation of lipase- producing bacteria in which different inducers (olive oil and Tween 80) used for enzyme production.
The effect of different pH and temperatures were investigated.
Fifteen lipase - producing bacteria had been isolated; Pseudomonas aeruginosa (6 isolates), Escherichia coli (5 isolates), Klebsiella pneumonia (3 isolates) and Staphylococcus epidermidis (1 isolate).
Olive oil was found to be the more suitable source of carbon and lipid than Tween 80 for lipase- producing bacteria.
All lipase- producing bacteria were capable to grow at different pH except of E.coli and S.
epidermidis which could not grow at pH 9.
All lipase- producing bacteria were capable to grow at different temperature except of E.coli, K.pneumoniae and S.
epidermidis could not grow at 4ᴏC.
P.
aeruginosa was considered the best lipase- producing bacteria which could handle the different conditions, and the optimum conditions for growth and enzyme production was at 37 ᴏC, pH 7 and incubation period 48hrs.
or more.
American Psychological Association (APA)
Muhsin, Yusra M. B.& al-Kaki, Sana Nuri Husayn& Baqir, Batul Abd al-Amir& Hamid, Inam& Ubayd, Khitam Ali& al-Kabi, Sadiq Abd al-Rida Qatia. 2016. Optimization and characterization growth conditions of lipase-producing bacteria from oil contaminated soil and sewage. Iraqi Journal of Biotechnology،Vol. 15, no. 3, pp.65-70.
https://search.emarefa.net/detail/BIM-769536
Modern Language Association (MLA)
Muhsin, Yusra M. B.…[et al.]. Optimization and characterization growth conditions of lipase-producing bacteria from oil contaminated soil and sewage. Iraqi Journal of Biotechnology Vol. 15, no. 3 (2016), pp.65-70.
https://search.emarefa.net/detail/BIM-769536
American Medical Association (AMA)
Muhsin, Yusra M. B.& al-Kaki, Sana Nuri Husayn& Baqir, Batul Abd al-Amir& Hamid, Inam& Ubayd, Khitam Ali& al-Kabi, Sadiq Abd al-Rida Qatia. Optimization and characterization growth conditions of lipase-producing bacteria from oil contaminated soil and sewage. Iraqi Journal of Biotechnology. 2016. Vol. 15, no. 3, pp.65-70.
https://search.emarefa.net/detail/BIM-769536
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 70
Record ID
BIM-769536