Direct detection of Clostridium perfringens based alpha toxin gene from sheep and cattle by real time pcr technique

الملخص EN

Real-time polymerase chain reaction assay was used for detection of Clostridium perfringens toxins genes alpha (cpa )directly from the fecal samples of cattle and sheep.Fecal samples from 20 clinically healthy cattle and 20 clinically healthy sheep were collected randomly from different farms located in ALDiwanyia city ,All the strains of Clostridium perfringens were analyzed by Real- Time PCR using specific primers for alpha (cpa)toxin genes ,its reveald that (cpa)toxin gene of Clostridium perfringens ,were detected in cattles in 8(40%) and 14 (70%) in sheep Real time positive respectively samples ,This results indicate that Clostridium perfringens alpha toxin producing strains were prevalent in sheep and cattles and its possibly play an important role in the diarrheal disease caused by Clostridium perfringens