Isolation and identification of alocalstrain of probiotic bacterial lactobacillus plantarum and studied the tolerance ability for different levels of pH
Other Title(s)
عزل و تشخيص البكتريا العلاجية Lactobacillus plantarum IRQ12 من المخلل المحلي و دراسة قابلية تحملها لمستويات مختلفة من الرقم الهيدروجيني
Joint Authors
al-Sharifi, Hasan Rahim
Abd al-Abbas, Sinan Jawdah
al-Bayar, Aswan Hamad Allah Abbud
al-Badran, Adnan Isa
Source
Basrah Journal of Veterinary Research
Issue
Vol. 15, Issue 2 (31 Dec. 2016), pp.329-345, 17 p.
Publisher
University of Basrah College of Veterinary Medicine
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
17
Main Subjects
Topics
Abstract EN
Lactobacillus plantarum IRQ12 was isolated from local pickle producing from Cucumis sativus submerged in 5% salt solution for one week far from sun light, selective media was used for isolation and activation process which was MRS broth and MRS agar, biochemical tests like catalase, nitrate reducing, productive gas, citrate consumption, produced ammonia from arginine, produced indole from tryptophan, fluid gelatin, ability of growth in 15, 45 °C, carbohydrates fermentation test, were carried out.
DNA of bacteria was extracted by DNA extraction kit, identification by 16S rDNA gene.
the local strain tolerance ability was investigated for different levels of pH 4, 3, 2 and 1.5 for 0, 1, 2 and 3 hours, log cfu/ml at pH 4 after 3 hours of treatment was 7.83 and at pH 3 the log cfu/ml decrease to 6.8 after 3 hours of treatment.
The log cfu/ml was decreased to 5.76 and 5.05 at pH 2 and 1.5 respectively after 3 hours of treatment.
American Psychological Association (APA)
Abd al-Abbas, Sinan Jawdah& al-Badran, Adnan Isa& al-Bayar, Aswan Hamad Allah Abbud& al-Sharifi, Hasan Rahim. 2016. Isolation and identification of alocalstrain of probiotic bacterial lactobacillus plantarum and studied the tolerance ability for different levels of pH. Basrah Journal of Veterinary Research،Vol. 15, no. 2, pp.329-345.
https://search.emarefa.net/detail/BIM-779017
Modern Language Association (MLA)
al-Badran, Adnan Isa…[et al.]. Isolation and identification of alocalstrain of probiotic bacterial lactobacillus plantarum and studied the tolerance ability for different levels of pH. Basrah Journal of Veterinary Research Vol. 15, no. 2 (2016), pp.329-345.
https://search.emarefa.net/detail/BIM-779017
American Medical Association (AMA)
Abd al-Abbas, Sinan Jawdah& al-Badran, Adnan Isa& al-Bayar, Aswan Hamad Allah Abbud& al-Sharifi, Hasan Rahim. Isolation and identification of alocalstrain of probiotic bacterial lactobacillus plantarum and studied the tolerance ability for different levels of pH. Basrah Journal of Veterinary Research. 2016. Vol. 15, no. 2, pp.329-345.
https://search.emarefa.net/detail/BIM-779017
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 341-345
Record ID
BIM-779017