Direct detection and identification of mycobacteria in bovine samples by PCR assay
Joint Authors
Nasr, Isam Amin
Hasan, Walid Hamdi
Musa, Hasan Muhammad
Source
Journal of Veterinary Medical Research
Issue
Vol. 24, Issue 1 (30 Jun. 2017)7 p.
Publisher
Beni-Suef University Faculty of Veterinary Medicine
Publication Date
2017-06-30
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Bovine tuberculosis, caused by Mycobacterium bovis, is a zoonotic disease causing approximately 6% of total human deaths.
Its economic losses are not only a reduction of 10-20% in milk production and weight, but also infertility and condemnation of meat.
Many serological tests are applied for detection of tuberculosis.
ELISA test has the highest sensitivity and specificity than the other serological tests for the diagnosis of tuberculosis.
Several forms of new technology were brought into the diagnostic approach to mycobacterial infection.
The aim of this work was to detect bovine tuberculosis by application of different traditional tests and PCR.
Tuberculin skin test was applied on 2650 cattle, only 63(2.4%) were positive.
Forty eight (76.2%) of the slaughtered positive animals showed visible lesions (VL) while the other 15 (23.8%) had non-visible lesions (NVL).
Bacteriological examination of 10 selected tuberculin positive samples revealed M.
bovis from 6 processed samples (60%) while PCR and ELISA assays revealed positive in 8 cases (80%) and 7 cases (70%), respectively.
It was concluded that PCR test is more sensitive and specific test to confirm the infection with tuberculosis
American Psychological Association (APA)
Hasan, Walid Hamdi& Nasr, Isam Amin& Musa, Hasan Muhammad. 2017. Direct detection and identification of mycobacteria in bovine samples by PCR assay. Journal of Veterinary Medical Research،Vol. 24, no. 1.
https://search.emarefa.net/detail/BIM-787880
Modern Language Association (MLA)
Nasr, Isam Amin…[et al.]. Direct detection and identification of mycobacteria in bovine samples by PCR assay. Journal of Veterinary Medical Research Vol. 24, no. 1 (2017).
https://search.emarefa.net/detail/BIM-787880
American Medical Association (AMA)
Hasan, Walid Hamdi& Nasr, Isam Amin& Musa, Hasan Muhammad. Direct detection and identification of mycobacteria in bovine samples by PCR assay. Journal of Veterinary Medical Research. 2017. Vol. 24, no. 1.
https://search.emarefa.net/detail/BIM-787880
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-787880