تأثير نوع الجزء النباتي و بعض منظمات النمو في نشوء زروعات نبات Stevia خارج الجسم الحي
Other Title(s)
Effect of expalnt type and some plant growth regulators on culture initiation of Stevia plants in vitro
Joint Authors
العبيدي، علا موجد عبد الزهرة
خير الله، حسام سعد الدين محمد
Source
Issue
Vol. 48, Issue 5 (31 Oct. 2017), pp.1206-1214, 9 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2017-10-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
This research was conducted in order to study the effect of explant type and some plant growth regulators on culture initiation of Stevia rebaudiana Bertoni in vitro.
The experiments included surface sterilization and test two types of explants (shoot tips and stem nodes) and the impact of KIN and BA and IAA and IBA in the cultures initiation.
Results revealed the efficiency of sodium hypochlorite (NaOCl) for disinfestation of explant at 0.050% concentration giving less contamination for shoot tips and stem nods (10% and 20% respectively).
Results showed that shoot tips inoculated in MS medium plus KIN at 0.3 mg.
L-1 was significantly increase the number of regenerated shoots as it produced 4.2 shoots per explant while medium without cytokinin (control) produced less number of shoots reached 1.4 shoots per explant.
KIN treatment reduced shoots length as control treatment produced the highest length (6.74 cm).
The interaction between the explant type and BA concentration was significantly increase the number of regenerated shoots as shoot tips produced 3.6 shoots per explant in MS medium supplemented with 0.1 mg.
L-1.
BA treatment reduced shoots length as control treatment produced the highest length (6.74 cm).
No positive effect was gain when auxins (IBA and IAA) were added in combination with cytokinin in culture medium.
The above results can be adopted to established stevia in vitro culture successfully.
American Psychological Association (APA)
خير الله، حسام سعد الدين محمد والعبيدي، علا موجد عبد الزهرة. 2017. تأثير نوع الجزء النباتي و بعض منظمات النمو في نشوء زروعات نبات Stevia خارج الجسم الحي. مجلة العلوم الزراعية العراقية،مج. 48، ع. 5، ص ص. 1206-1214.
https://search.emarefa.net/detail/BIM-795996
Modern Language Association (MLA)
خير الله، حسام سعد الدين محمد والعبيدي، علا موجد عبد الزهرة. تأثير نوع الجزء النباتي و بعض منظمات النمو في نشوء زروعات نبات Stevia خارج الجسم الحي. مجلة العلوم الزراعية العراقية مج. 48، ع. 5 (2017)، ص ص. 1206-1214.
https://search.emarefa.net/detail/BIM-795996
American Medical Association (AMA)
خير الله، حسام سعد الدين محمد والعبيدي، علا موجد عبد الزهرة. تأثير نوع الجزء النباتي و بعض منظمات النمو في نشوء زروعات نبات Stevia خارج الجسم الحي. مجلة العلوم الزراعية العراقية. 2017. مج. 48، ع. 5، ص ص. 1206-1214.
https://search.emarefa.net/detail/BIM-795996
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 1213-1214
Record ID
BIM-795996