عزل و غربلة و تشخيص بكتريا Bacillus sp. المنتجة لأنزيم Alkaline protease
Other Title(s)
Isolation, screening and identification of Bacillus sp. producing alkaline protease
Joint Authors
Source
Issue
Vol. 48, Issue 6 (31 Dec. 2017), pp.1493-1503, 11 p.
Publisher
University of Baghdad College of Agriculture
Publication Date
2017-12-31
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Topics
Abstract EN
Twenty-five bacteria isolates (Bacillus sp.) were isolated from different local sources (soil of college of Agriculture/university of Baghdad and from Amiriya); the obtained isolates were screened for their capabilities for producing alkaline proteases.
It has been noticed that isolates designated (2,5,6,8,11,12 at 37°C) and (14,17 and 18 at 55°C) were showed high capability for producing alkaline protease.
All isolates subjected to secondary screening.
The results revealed that isolate (12) coded MH12 was the highest producers with enzyme activity of (81.00U/ml).
The Identification tests of this isolate were carried out by studying morphological, microscopic characteristic and using of Vitek2 compact system.
These tests were conformed by identification of 16S rRNA gene using PCR and its nitrogen base sequencing and the results revealed that the isolate was belongs to Bacillus cereus.
American Psychological Association (APA)
مصطفى محمد عمر وحميد عبود جبر. 2017. عزل و غربلة و تشخيص بكتريا Bacillus sp. المنتجة لأنزيم Alkaline protease. مجلة العلوم الزراعية العراقية،مج. 48، ع. 6، ص ص. 1493-1503.
https://search.emarefa.net/detail/BIM-812332
Modern Language Association (MLA)
مصطفى محمد عمر وحميد عبود جبر. عزل و غربلة و تشخيص بكتريا Bacillus sp. المنتجة لأنزيم Alkaline protease. مجلة العلوم الزراعية العراقية مج. 48، ع. 6 (2017)، ص ص. 1493-1503.
https://search.emarefa.net/detail/BIM-812332
American Medical Association (AMA)
مصطفى محمد عمر وحميد عبود جبر. عزل و غربلة و تشخيص بكتريا Bacillus sp. المنتجة لأنزيم Alkaline protease. مجلة العلوم الزراعية العراقية. 2017. مج. 48، ع. 6، ص ص. 1493-1503.
https://search.emarefa.net/detail/BIM-812332
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 1502-1503
Record ID
BIM-812332