The effect of using a new local oily inject able cadmium complex (CLC)‎ in layer hens'(Lohman Brown)‎ to control and salpingitis caused by (E. coli)‎ and hen day egg production

Abstract EN

An oily injectable administration CLC, chemically named [Dinitrato- bis {(p-methyl anilino) phenyl acetonitrile} Cadmium (II)].

(2) Hydrate, its chemical formula as [Cd (HL) 2(NO3)2].

2H2O was manufactured as a complex, and tested in vitro against E.coli (sensitivity test) was showed inhibitory zone of 12 mm(+++).The CLC was injected subcutaneously in the layer hens neck ( Lohman brown) at different levels ,T1 0.0% , T2 0.25%, T3 0.50% and(T4 ,T5)1.0%.After five days E.coli was orally administrated by drinking water at concentration of 8500x106cell ml, (highly pathogenic) as causative agent of Salpengitis and Ovaritis ,after another five days direct smears of E.coli from ovaries and oviducts , which had been cultured on MacConkey Red agar ( differential agar for E.coli diagnosis) to identify E.coli colonies , also it tested biochemically by( Indole &Methylene Red ) tests which were both + ve.

The Results after four months are revealed that the treated chickens showed a resistance against E.coli infection; moreover the bacterial counts results of this study are associated with significant decreases of E.coli counts as compared to normal control (T1), while there were significant increases in (T5: doesn’t inject CLC but administrated E.col orally by drinking water), that showed a pathological case.

When a pathological anatomy had been mead there was ascitis, gaseous ovary, atrophied oviduct which are the main pathological symptoms of ovaritis and Salpengitis.

The protective effects of CLC in respect to decreases E.coli counts in egg, ovaries and oviducts in T3 (0.050).

But chickens hen day showed significant decreases and the lowest egg production drop were in T3 (0.50%) followed by T2, T4 and T1 respectively.

Then confirmed by measuring the residual concentration of Cd in liver and egg of treated chickens by using the atomic absorption, which revealed that livers and egg are free from any Cd residues.

An oily injectable administration CLC, chemically named [Dinitrato- bis {(p-methyl anilino) phenyl acetonitrile} Cadmium (II)].

(2) Hydrate, its chemical formula as [Cd (HL) 2(NO3)2].

2H2O was manufactured as a complex, and tested in vitro against E.coli (sensitivity test) was showed inhibitory zone of 12 mm(+++).The CLC was injected subcutaneously in the layer hens neck ( Lohman brown) at different levels ,T1 0.0% , T2 0.25%, T3 0.50% and(T4 ,T5)1.0%.After five days E.coli was orally administrated by drinking water at concentration of 8500x106cell ml, (highly pathogenic) as causative agent of Salpengitis and Ovaritis ,after another five days direct smears of E.coli from ovaries and oviducts , which had been cultured on MacConkey Red agar ( differential agar for E.coli diagnosis) to identify E.coli colonies , also it tested biochemically by( Indole &Methylene Red ) tests which were both + ve.

The Results after four months are revealed that the treated chickens showed a resistance against E.coli infection; moreover the bacterial counts results of this study are associated with significant decreases of E.coli counts as compared to normal control (T1), while there were significant increases in (T5: doesn’t inject CLC but administrated E.col orally by drinking water), that showed a pathological case.

When a pathological anatomy had been mead there was ascitis, gaseous ovary, atrophied oviduct which are the main pathological symptoms of ovaritis and Salpengitis.

The protective effects of CLC in respect to decreases E.coli counts in egg, ovaries and oviducts in T3 (0.050).

But chickens hen day showed significant decreases and the lowest egg production drop were in T3 (0.50%) followed by T2, T4 and T1 respectively.

Then confirmed by measuring the residual concentration of Cd in liver and egg of treated chickens by using the atomic absorption, which revealed that livers and egg are free from any Cd residues.