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Isolation and Purification of CNF1 (Cytotoxic necrosis factor 1) produced from bacteria Escherichia coli and study its role against apoptosis in vitro
Other Title(s)
عزل وتنقيه 1 (CNF1 (Cytotoxic necrosis factorالمنتج من بكتيريا الأشريشية القولونية و دراسة دورة المضاد لعمليه الموت المبرمج خارج الجسم الحي
Joint Authors
Hasan, Muthana Hamid
al-Rikabi, Nahi Yusuf Yasin
Fulayyih, Mayy Talib
Source
Iraqi Journal of Cancer and Medical Genetics
Issue
Vol. 8, Issue 2 (31 Dec. 2015), pp.157-163, 7 p.
Publisher
al-Mustansyriah University Iraqi Centre for Cancer and Medical Genetics Research
Publication Date
2015-12-31
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
Through the period April - December 2012, urine specimens were collected from186 patiants attending five hospitals.
Seventy six isolates of uropathogenic E.
coli were obtained from these samples.
These isolates were identified as E.
coli according to the cultural and morphological characteristics on MacConkey agar, eosin methylene blue agar, biochemical test (vitek 2 system), and API 20 E system.
The results showed that only four E.
coli isolates (E4, E8, E23, E36) produced cytotoxic necrosis factor 1(CNF1), and E23 was the more efficient isolate in CNF1 production and have high activity on tissue culture.
CNF1 produced from this isolate was purified by two steps; the first was by ion exchange chromatography using DEAESephadex, and the second step was gel filtration by Sephacryl S-100.
Also study Polymerase Chain Reaction (PCR) for cnf1 gene and used primer cnf1.
The size of amplified gene was 543pb.
The cytotoxic effect of different concentrations of CNF1 on cancer lines (RD, AMN3, , Hela, PC3 and HePG2) at 72 hr were examined .The effect was manifested multinucleated and enlargement in cell line.
The low IC50 was 104μg/ml against HePG2cancer cells after 72 hr.
Also study multiparameter cytotoxic assay.
The results revealed low concentration of cytochrome c, total nuclear intensity, cell membrane permeability in comparative positive control.
The mitochondria concentration was high compared to the positive control on of cell lines in the concentration (100,25, 12.5,6.25).
American Psychological Association (APA)
Hasan, Muthana Hamid& Fulayyih, Mayy Talib& al-Rikabi, Nahi Yusuf Yasin. 2015. Isolation and Purification of CNF1 (Cytotoxic necrosis factor 1) produced from bacteria Escherichia coli and study its role against apoptosis in vitro. Iraqi Journal of Cancer and Medical Genetics،Vol. 8, no. 2, pp.157-163.
https://search.emarefa.net/detail/BIM-819403
Modern Language Association (MLA)
Hasan, Muthana Hamid…[et al.]. Isolation and Purification of CNF1 (Cytotoxic necrosis factor 1) produced from bacteria Escherichia coli and study its role against apoptosis in vitro. Iraqi Journal of Cancer and Medical Genetics Vol. 8, no. 2 (2015), pp.157-163.
https://search.emarefa.net/detail/BIM-819403
American Medical Association (AMA)
Hasan, Muthana Hamid& Fulayyih, Mayy Talib& al-Rikabi, Nahi Yusuf Yasin. Isolation and Purification of CNF1 (Cytotoxic necrosis factor 1) produced from bacteria Escherichia coli and study its role against apoptosis in vitro. Iraqi Journal of Cancer and Medical Genetics. 2015. Vol. 8, no. 2, pp.157-163.
https://search.emarefa.net/detail/BIM-819403
Data Type
Journal Articles
Language
English
Notes
Record ID
BIM-819403